Mla- and Rom1-mediated control of microRNA398 and chloroplast copper/zinc superoxide dismutase regulates cell death in response to the barley powdery mildew fungus
No claim to original US Government works. New Phytologist © 2013 New Phytologist Trust.
| Veröffentlicht in: | The New phytologist. - 1979. - 201(2014), 4 vom: 18. März, Seite 1396-1412 |
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| Weitere Verfasser: | , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, Non-P.H.S. Blumeria graminis barley hypersensitive reaction microRNA reactive oxygen species (ROS) superoxide dismutase (SOD) MicroRNAs Plant Proteins mehr... |
| Zusammenfassung: | No claim to original US Government works. New Phytologist © 2013 New Phytologist Trust. • Barley (Hordeum vulgare L.) Mildew resistance locus a (Mla) confers allele-specific interactions with natural variants of the ascomycete fungus Blumeria graminis f. sp. hordei (Bgh), the causal agent of powdery mildew disease. Significant reprogramming of Mla-mediated gene expression occurs upon infection by this obligate biotrophic pathogen. • We utilized a proteomics-based approach, combined with barley mla, required for Mla12 resistance1 (rar1), and restoration of Mla resistance1 (rom1) mutants, to identify components of Mla-directed signaling. • Loss-of-function mutations in Mla and Rar1 both resulted in the reduced accumulation of chloroplast copper/zinc superoxide dismutase 1 (HvSOD1), whereas loss of function in Rom1 re-established HvSOD1 levels. In addition, both Mla and Rom1 negatively regulated hvu-microRNA398 (hvu-miR398), and up-regulation of miR398 was coupled to reduced HvSOD1 expression. Barley stripe mosaic virus (BSMV)-mediated over-expression of both barley and Arabidopsis miR398 repressed accumulation of HvSOD1, and BSMV-induced gene silencing of HvSod1 impeded Mla-triggered H₂O₂ and hypersensitive reaction (HR) at barley-Bgh interaction sites. • These data indicate that Mla- and Rom1-regulated hvu-miR398 represses HvSOD1 accumulation, influencing effector-induced HR in response to the powdery mildew fungus |
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| Beschreibung: | Date Completed 29.09.2014 Date Revised 17.04.2021 published: Print-Electronic GENBANK: JQ277734, JQ364431, JQ364432, JQ364433, JQ364434, JQ364435, JQ364436, JQ364437, JQ364438, JQ364439, JQ364440, JQ364441, JQ364442, JQ364443, JQ364444, JQ364445, JQ364446, JQ364447, JQ364448, JQ364449, JQ364450, JQ364451, JQ364452, JQ364453, JQ364454 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.12598 |