Characterization of the response of in vitro cultured Myrtus communis L. plants to high concentrations of NaCl

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 73(2013) vom: 13. Dez., Seite 420-6
1. Verfasser:	Di Cori, P (VerfasserIn)
Weitere Verfasser:	Lucioli, S, Frattarelli, A, Nota, P, Tel-Or, E, Benyamini, E, Gottlieb, H, Caboni, E, Forni, C
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article 6-benzylaminopurine APX BAP Enzymatic and non-enzymatic antioxidants G-POD IBA In vitro culture LN MDA mehr... MKI McKinney Index Myrtle PCD ROS Salt stress ascorbate peroxidase f.w. fresh weight guaiacol peroxidase indole-3-butyric acid liquid nitrogen malondialdehyde programmed cell death reactive oxygen species Culture Media Reactive Oxygen Species Chlorophyll 1406-65-1 Carotenoids 36-88-4 Sodium Chloride 451W47IQ8X Arginine 94ZLA3W45F Proline 9DLQ4CIU6V EC 1.11.1.- Ascorbate Peroxidases EC 1.11.1.11 Peroxidase EC 1.11.1.7 Superoxide Dismutase EC 1.15.1.1 Cysteine K848JZ4886

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier Masson SAS. All rights reserved. Effect of salt stress was examined in in vitro shoot cultures of Myrtus communis L. a species of the Mediterranean maquis. To determine the effects of high salt concentrations on myrtle plantlets and contribute toward understanding the mechanisms adopted from this species to counteract soil salinity, in vitro rooted shoots were transferred to a liquid culture medium containing 0, 125 or 250 mM NaCl for 30 days. After 15 and 30 days of in vitro culture, shoot and root growth, chlorosis and necrosis extension, chlorophylls, carotenoids, proline, arginine, cysteine and total sugars content, as well as guaiacol peroxidase (G-POD, EC 1.11.1.7) and ascorbate peroxidase (APX, EC 1.11.1.11) activities were determined. In treated plants shoot and root growth, as well as chlorophyll content, significantly decreased, while carotenoids content was not affected by the NaCl treatment. Among osmolytes, proline did not significantly increase, arginine and cysteine decreased, while total sugars were found to be higher in the treated plants than in the control. Enhancement of G-POD and APX activities was positively related to increasing salt concentrations in the culture media, regardless of the exposure time. Salt-treated plants did not show significant changes in lipid peroxidation or DNA fragmentation after 30 days salt treatment, regardless of the NaCl concentrations applied. The results represent a contribution towards understanding the mechanisms adopted by this species to high salinity
Beschreibung:	Date Completed 29.07.2014 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.10.026