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20231224092952.0 |
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cr uuu---uuuuu |
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231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1111/j.1365-2761.2012.01424.x
|2 doi
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| 028 |
5 |
2 |
|a pubmed24n0774.xml
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|a (DE-627)NLM232434530
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| 035 |
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|a (NLM)24206018
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| 040 |
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Ho, L-P
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Evaluating the protective efficacy of antigen combinations against Photobacterium damselae ssp. piscicida infections in cobia, Rachycentron canadum L
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| 264 |
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1 |
|c 2014
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 14.05.2014
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| 500 |
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|a Date Revised 30.09.2020
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| 500 |
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|a published: Print-Electronic
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a © 2013 John Wiley & Sons Ltd.
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| 520 |
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|a Cobia, Rachycentron canadum L., is a very important aquatic fish that faces the risk of infection with the bacterial pathogen Photobacterium damselae ssp. piscicida, and there are few protective approaches available that use multiple antigens. In the present study, potent bivalent antigens from P. damselae ssp. piscicida showed more efficient protection than did single antigens used in isolation. In preparations of three antigens that included recombinant heat shock protein 60 (rHSP60), recombinant α-enolase (rENOLASE) and recombinant glyceraldehyde-3-phosphate dehydrogenase (rGAPDH), we analysed the doses that elicited the best immune responses and found that this occurred at a total of 30 μg of antigen per fish. Subsequently, vaccination of fish with rHSP60, rENOLASE and rGAPDH achieved 46.9, 52 and 25% relative per cent survival (RPS), respectively. In addition, bivalent subunit vaccines--combination I (rHSP60 + rENOLASE), combination II (rENOLASE + rGAPDH) and combination III (rHSP60 + rGAPDH)--were administered and the RPS in these groups (65.6, 64.0 and 48.4%, respectively), was higher than that achieved with single-antigen administration. Finally, in combination IV, the trivalent vaccine rHSP60 + rENOLASE + rGAPDH, the RPS was 1.6%. Taken together, our results suggest that combinations of two antigens may achieve a better efficiency than monovalent or trivalent antigens, and this may provide new insights into pathogen prevention strategies
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Photobacterium damselae ssp. piscicida
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| 650 |
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4 |
|a bacterial pathogen
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| 650 |
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4 |
|a bivalent subunit vaccine
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| 650 |
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4 |
|a synergetic effect
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| 650 |
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4 |
|a vaccination technology
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| 650 |
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7 |
|a Antibodies, Bacterial
|2 NLM
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| 650 |
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7 |
|a Antigens, Bacterial
|2 NLM
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| 650 |
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7 |
|a Bacterial Vaccines
|2 NLM
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| 700 |
1 |
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|a Chang, C-J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, H-C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, H-L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, J H-Y
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 37(2014), 1 vom: 23. Jan., Seite 51-62
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
8 |
|g volume:37
|g year:2014
|g number:1
|g day:23
|g month:01
|g pages:51-62
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/j.1365-2761.2012.01424.x
|3 Volltext
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 37
|j 2014
|e 1
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|c 01
|h 51-62
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