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01000caa a22002652 4500 |
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NLM232310610 |
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DE-627 |
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20240323232705.0 |
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cr uuu---uuuuu |
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231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1093/jxb/ert361
|2 doi
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| 028 |
5 |
2 |
|a pubmed24n1342.xml
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|a (DE-627)NLM232310610
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| 035 |
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|a (NLM)24192995
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| 040 |
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Ben Amar, Siwar
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Functional characterization in Xenopus oocytes of Na+ transport systems from durum wheat reveals diversity among two HKT1;4 transporters
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| 264 |
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1 |
|c 2014
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 26.09.2014
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| 500 |
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|a Date Revised 23.03.2024
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|a published: Print-Electronic
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| 500 |
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|a GENBANK: KF443078, KF443079
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a Plant tolerance to salinity constraint involves complex and integrated functions including control of Na(+) uptake, translocation, and compartmentalization. Several members of the high-affinity K(+) transporter (HKT) family, which comprises plasma-membrane transporters permeable to K(+) and Na(+) or to Na(+) only, have been shown to play major roles in plant Na(+) and K(+) homeostasis. Among them, HKT1;4 has been identified as corresponding to a quantitative trait locus (QTL) of salt tolerance in wheat but was not functionally characterized. Here, we isolated two HKT1;4-type cDNAs from a salt-tolerant durum wheat (Triticum turgidum L. subsp. durum) cultivar, Om Rabia3, and investigated the functional properties of the encoded transporters using a two-electrode voltage-clamp technique, after expression in Xenopus oocytes. Both transporters displayed high selectivity for Na(+), their permeability to other monovalent cations (K(+), Li(+), Cs(+), and Rb(+)) being ten times lower than that to Na(+). Both TdHKT1;4-1 and TdHKT1;4-2 transported Na(+) with low affinity, although the half-saturation of the conductance was observed at a Na(+) concentration four times lower in TdHKT1;4-1 than in TdHKT1;4-2. External K(+) did not inhibit Na(+) transport through these transporters. Quinine slightly inhibited TdHKT1;4-2 but not TdHKT1;4-1. Overall, these data identified TdHKT1;4 transporters as new Na(+)-selective transporters within the HKT family, displaying their own functional features. Furthermore, they showed that important differences in affinity exist among durum wheat HKT1;4 transporters. This suggests that the salt tolerance QTL involving HKT1;4 may be at least in part explained by functional variability among wheat HKT1;4-type transporters
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Durum wheat
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| 650 |
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4 |
|a HKT1;4
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| 650 |
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4 |
|a Xenopus oocyte.
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| 650 |
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4 |
|a electrophysiology
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| 650 |
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4 |
|a salt tolerance
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| 650 |
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4 |
|a sodium transport
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| 650 |
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7 |
|a Cation Transport Proteins
|2 NLM
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| 650 |
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7 |
|a Cations
|2 NLM
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| 650 |
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7 |
|a DNA, Complementary
|2 NLM
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| 650 |
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7 |
|a DNA, Plant
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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7 |
|a Protein Isoforms
|2 NLM
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| 650 |
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7 |
|a Sodium
|2 NLM
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| 650 |
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7 |
|a 9NEZ333N27
|2 NLM
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| 700 |
1 |
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|a Brini, Faiçal
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sentenac, Hervé
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Masmoudi, Khaled
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Véry, Anne-Aliénor
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 1 vom: 06. Jan., Seite 213-22
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:65
|g year:2014
|g number:1
|g day:06
|g month:01
|g pages:213-22
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| 856 |
4 |
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|u http://dx.doi.org/10.1093/jxb/ert361
|3 Volltext
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 65
|j 2014
|e 1
|b 06
|c 01
|h 213-22
|