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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2013.10.018
|2 doi
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|a pubmed24n0774.xml
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|a (DE-627)NLM232229023
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|a (NLM)24184455
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|a (PII)S0981-9428(13)00368-9
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Afitlhile, Meshack
|e verfasserin
|4 aut
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|a A mutant of the Arabidopsis thaliana TOC159 gene accumulates reduced levels of linolenic acid and monogalactosyldiacylglycerol
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 29.07.2014
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier Masson SAS. All rights reserved.
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|a Previous studies have shown that a mutant of Arabidopsis that lacks the Toc159 receptor is impaired in chloroplast biogenesis. The mutant is referred as plastid protein import 2 or ppi2 and has an albino phenotype due to its inability to import the photosynthetic proteins. In this study, we measured fatty acid composition and transcript levels of plastid-localized fatty acid desaturases in the wild type and ppi2 mutant. The objective was to evaluate whether the Toc159 receptor was critical in the import of lipid-synthesizing enzymes. The ppi2 mutant accumulated decreased levels of oleic acid (18:1) and α-linolenic acid (18:3). The mutant accumulated drastically reduced amounts of the chloroplast lipid monogalactosyldiacylglycerol (MGDG), which contains more than 80% of 18:3. The expression of genes that encode stearoyl-ACP desaturase and MGD1 synthase were down-regulated in the ppi2 mutant, and this corresponded to decreased levels of 18:1 and MGDG, respectively. We conclude that in the ppi2 mutant the impaired synthesis of MGDG resulted in decreased amounts of 18:3. The mutant however, had a 30-fold increase in fad5 transcript levels; this increase was mirrored by a 16- to 50-fold accumulation of hexadecatrienoic acid (16:3), a fatty acid found exclusively in MGDG. Taken together, these data suggest that the Toc159 receptor is required in the import of stearoyl-ACP desaturase and MGD1 synthase into the chloroplasts. Since the expression of fad5 gene was up-regulated in the ppi2 mutant, we propose that fad5 desaturase is imported into plastids through the atToc132/atToc120 protein import pathway
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a 16:3
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|a 18:3
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|a Arabidopsis thaliana
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|a Fad
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|a Fad5
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|a MGD1 synthase
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|a MGDG
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|a TOC
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|a fatty acid desaturases
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|a hexadecatrienoic acid
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|a monogalactosyldiacylglycerol
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|a plastid protein import 2
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|a ppi2
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|a translocon at the outer envelope of chloroplasts
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|a α-linolenic acid
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|a Arabidopsis Proteins
|2 NLM
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|a Galactolipids
|2 NLM
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|a Membrane Proteins
|2 NLM
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|a Receptors, Cell Surface
|2 NLM
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|a TOC159 protein, Arabidopsis
|2 NLM
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|a Toc120 protein, Arabidopsis
|2 NLM
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| 650 |
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|a monogalactosyldiacylglycerol
|2 NLM
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| 650 |
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|a alpha-Linolenic Acid
|2 NLM
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| 650 |
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|a 0RBV727H71
|2 NLM
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| 650 |
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|a Oleic Acid
|2 NLM
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|a 2UMI9U37CP
|2 NLM
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| 650 |
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|a Fatty Acid Desaturases
|2 NLM
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| 650 |
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|a EC 1.14.19.-
|2 NLM
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| 650 |
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|a Galactosyltransferases
|2 NLM
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| 650 |
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|a EC 2.4.1.-
|2 NLM
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| 650 |
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|a 1,2-diacylglycerol 3-beta-galactosyltransferase
|2 NLM
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| 650 |
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|a EC 2.4.1.46
|2 NLM
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| 650 |
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|a GTP Phosphohydrolases
|2 NLM
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| 650 |
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|a EC 3.6.1.-
|2 NLM
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|a Workman, Samantha
|e verfasserin
|4 aut
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|a Duffield, Kayla
|e verfasserin
|4 aut
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|a Sprout, Danielle
|e verfasserin
|4 aut
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| 700 |
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|a Berhow, Mark
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 73(2013) vom: 15. Dez., Seite 344-50
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:73
|g year:2013
|g day:15
|g month:12
|g pages:344-50
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|u http://dx.doi.org/10.1016/j.plaphy.2013.10.018
|3 Volltext
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|a SYSFLAG_A
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|a GBV_ILN_350
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|a AR
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|d 73
|j 2013
|b 15
|c 12
|h 344-50
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