Identification and characterization of (E)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton

Identification and characterization of (E)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 73(2013) vom: 15. Dez., Seite 302-8
1. Verfasser: Huang, Xinzheng (VerfasserIn)
Weitere Verfasser: Xiao, Yutao, Köllner, Tobias G, Zhang, Wanna, Wu, Junxiang, Wu, Juan, Guo, Yuyuan, Zhang, Yongjun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Apolygus lucorum Methyl jasmonate Plant defense Quantitative real-time PCR α-Pinene β-Caryophyllene Acetates Bicyclic Monoterpenes mehr... Bridged Bicyclo Compounds Cyclopentanes Monocyclic Sesquiterpenes Monoterpenes Oxylipins Plant Proteins Polycyclic Sesquiterpenes Polyisoprenyl Phosphates Sesquiterpenes Terpenes beta-pinene 4MS8VHZ1HJ humulene 54W56MD2WD geranyl pyrophosphate 763-10-0 farnesyl pyrophosphate 79W6B01D07 methyl jasmonate 900N171A0F caryophyllene BHW853AU9H Alkyl and Aryl Transferases EC 2.5.- terpene synthase EC 2.5.1.- Carbon-Oxygen Lyases EC 4.2.- (-)-alpha-pinene synthase EC 4.2.3.119 alpha-pinene JPF3YI7O34
Beschreibung
Zusammenfassung:Copyright © 2013 Elsevier Masson SAS. All rights reserved.
Cotton (Gossypium hirsutum L.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. To understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, GhTPS1 and GhTPS2, were heterologously expressed and characterized. Recombinant GhTPS1 accepted farnesyl pyrophosphate as substrate and produced (E)-β-caryophyllene and α-humulene. GhTPS2 was characterized as a monoterpene synthase which formed α-pinene and β-pinene using geranyl pyrophosphate as substrate. Quantitative real-time PCR analysis revealed that GhTPS1 and GhTPS2 gene expression was elevated after methyl jasmonate (MeJA) treatment in cotton leaves. Moreover, feeding of the green plant bug Apolygus lucorum, a major cotton pest in northern China, resulted in increased GhTPS2 expression in young leaves, suggesting that GhTPS2 might be involved in plant defense in cotton
Beschreibung:Date Completed 29.07.2014
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2013.10.017