Dissimilar effects of β-lapachone- and hydroxyurea-induced DNA replication stress in root meristem cells of Allium cepa

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 73(2013) vom: 15. Dez., Seite 282-93
1. Verfasser: Zabka, Aneta (VerfasserIn)
Weitere Verfasser: Trzaskoma, Paweł, Maszewski, Janusz
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Cell cycle checkpoints DNA damage Hydrogen peroxide Hydroxyurea β-Lapachone γ-H2AX histones Chromatin mehr... DNA, Plant DNA-Binding Proteins H2AX protein, human Histones Mutagens Naphthoquinones Plant Extracts beta-lapachone 6N4FA2QQ6A Hydrogen Peroxide BBX060AN9V X6Q56QN5QC
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245 1 0 |a Dissimilar effects of β-lapachone- and hydroxyurea-induced DNA replication stress in root meristem cells of Allium cepa 
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520 |a Two anticancer drugs, β-lapachone (β-lap, a naphthoquinone) and hydroxyurea (HU, an inhibitor of ribonucleotide reductase), differently affect nuclear morphology and cell cycle control mechanisms in root meristem cells of Allium cepa. The 18 h treatment with 100 μM β-lap results in a lowered number of M-phase cells, increased occurrence of mitotic abnormalities, including over-condensation of chromosomes, their enhanced stickiness, formation of anaphase bridges, micronucleation and reduced mitotic spindles. Following prolonged incubations using high doses of β-lap, cell nuclei reveal dark-red fluorescence evenly distributed in chromatin surrounding the unstained regions of nucleoli. Both drugs generate H2O2 and induce DNA double strand breaks, which is correlated with γ-phoshorylation of H2AX histones. However, the extent of H2AX phosphorylation (including the frequency of γ-H2AX foci and the relative number cells creating phospho-H2AX domains) is considerably reduced in root meristem cells treated jointly with the β-lap/HU mixture. Furthermore, various effects of caffeine (an inhibitor of ATM/ATR cell cycle checkpoint kinases) on β-lap- and HU-induced γ-phoshorylation of H2AX histones and the protective activity of HU against β-lap suggest that their genotoxic activities are largely dissimilar. β-Lap treatment results in the induction of apoptosis-like programmed cell death, while HU treatment leads to cell adaptation to replication stress and promotion of abnormal nuclear divisions with biphasic interphase/mitotic states of chromatin condensation 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Cell cycle checkpoints 
650 4 |a DNA damage 
650 4 |a Hydrogen peroxide 
650 4 |a Hydroxyurea 
650 4 |a β-Lapachone 
650 4 |a γ-H2AX histones 
650 7 |a Chromatin  |2 NLM 
650 7 |a DNA, Plant  |2 NLM 
650 7 |a DNA-Binding Proteins  |2 NLM 
650 7 |a H2AX protein, human  |2 NLM 
650 7 |a Histones  |2 NLM 
650 7 |a Mutagens  |2 NLM 
650 7 |a Naphthoquinones  |2 NLM 
650 7 |a Plant Extracts  |2 NLM 
650 7 |a beta-lapachone  |2 NLM 
650 7 |a 6N4FA2QQ6A  |2 NLM 
650 7 |a Hydrogen Peroxide  |2 NLM 
650 7 |a BBX060AN9V  |2 NLM 
650 7 |a Hydroxyurea  |2 NLM 
650 7 |a X6Q56QN5QC  |2 NLM 
700 1 |a Trzaskoma, Paweł  |e verfasserin  |4 aut 
700 1 |a Maszewski, Janusz  |e verfasserin  |4 aut 
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773 1 8 |g volume:73  |g year:2013  |g day:15  |g month:12  |g pages:282-93 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2013.10.001  |3 Volltext 
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