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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2013.10.001
|2 doi
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|a pubmed24n0774.xml
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|a (DE-627)NLM232228957
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|a (NLM)24184448
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|a (PII)S0981-9428(13)00347-1
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Zabka, Aneta
|e verfasserin
|4 aut
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|a Dissimilar effects of β-lapachone- and hydroxyurea-induced DNA replication stress in root meristem cells of Allium cepa
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 29.07.2014
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|a Date Revised 18.07.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier Masson SAS. All rights reserved.
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|a Two anticancer drugs, β-lapachone (β-lap, a naphthoquinone) and hydroxyurea (HU, an inhibitor of ribonucleotide reductase), differently affect nuclear morphology and cell cycle control mechanisms in root meristem cells of Allium cepa. The 18 h treatment with 100 μM β-lap results in a lowered number of M-phase cells, increased occurrence of mitotic abnormalities, including over-condensation of chromosomes, their enhanced stickiness, formation of anaphase bridges, micronucleation and reduced mitotic spindles. Following prolonged incubations using high doses of β-lap, cell nuclei reveal dark-red fluorescence evenly distributed in chromatin surrounding the unstained regions of nucleoli. Both drugs generate H2O2 and induce DNA double strand breaks, which is correlated with γ-phoshorylation of H2AX histones. However, the extent of H2AX phosphorylation (including the frequency of γ-H2AX foci and the relative number cells creating phospho-H2AX domains) is considerably reduced in root meristem cells treated jointly with the β-lap/HU mixture. Furthermore, various effects of caffeine (an inhibitor of ATM/ATR cell cycle checkpoint kinases) on β-lap- and HU-induced γ-phoshorylation of H2AX histones and the protective activity of HU against β-lap suggest that their genotoxic activities are largely dissimilar. β-Lap treatment results in the induction of apoptosis-like programmed cell death, while HU treatment leads to cell adaptation to replication stress and promotion of abnormal nuclear divisions with biphasic interphase/mitotic states of chromatin condensation
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cell cycle checkpoints
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|a DNA damage
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|a Hydrogen peroxide
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|a Hydroxyurea
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|a β-Lapachone
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|a γ-H2AX histones
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|a Chromatin
|2 NLM
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|a DNA, Plant
|2 NLM
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|a DNA-Binding Proteins
|2 NLM
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|a H2AX protein, human
|2 NLM
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|a Histones
|2 NLM
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|a Mutagens
|2 NLM
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|a Naphthoquinones
|2 NLM
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|a Plant Extracts
|2 NLM
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|a beta-lapachone
|2 NLM
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|a 6N4FA2QQ6A
|2 NLM
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|a Hydrogen Peroxide
|2 NLM
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|a BBX060AN9V
|2 NLM
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|a Hydroxyurea
|2 NLM
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|a X6Q56QN5QC
|2 NLM
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|a Trzaskoma, Paweł
|e verfasserin
|4 aut
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|a Maszewski, Janusz
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 73(2013) vom: 15. Dez., Seite 282-93
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:73
|g year:2013
|g day:15
|g month:12
|g pages:282-93
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|u http://dx.doi.org/10.1016/j.plaphy.2013.10.001
|3 Volltext
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|a AR
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|d 73
|j 2013
|b 15
|c 12
|h 282-93
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