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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2013.08.009
|2 doi
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|a pubmed25n0773.xml
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|a (DE-627)NLM231983476
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|a (NLM)24157205
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|a (PII)S0168-9452(13)00183-0
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Rocha, Antônio J
|e verfasserin
|4 aut
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|a Differential expression of cysteine peptidase genes in the inner integument and endosperm of developing seeds of Jatropha curcas L. (Euphorbiaceae)
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 13.05.2014
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|a Date Revised 10.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
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|a In several plant tissues, programmed cell death (PCD) is mediated by the combined action of cysteine peptidases, namely KDEL-tailed cysteine peptidases (KDEL-CysEP) and vacuolar processing enzymes (VPE). Here, we performed a search of the draft genome of Jatropha curcas L. (Euphorbiaceae) and identified 2 genes for KDEL-CysEP (Jc-CysEP1 and Jc-CysEP2) and 3 genes for VPE (Jc-βVPE, Jc-γVPE and Jc-δVPE) and determined the expression patterns of these genes by RT-qPCR in integument and cellular endosperm of seeds collected at seven different developmental stages. We were able to demonstrate that the expression of Jc-CysEP1, Jc-CysEP2, Jc-βVPE and Jc-γVPE proceeded rapidly from Stage IV, with Jc-CysEP2 displaying the highest relative expression; expression of Jc-δVPE could not be detected in any of the tissues/developmental stages analyzed. Additionally, we showed that the expression pattern of these peptidases correlates with anatomical changes in integument and cellular endosperm, thus suggesting a role for both classes of peptidases in PCD and in protein processing, both of which occur simultaneously in each of these tissues
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a BLAST
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|a EST
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|a KDEL-CysEP
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|a KDEL-tailed cysteine peptidase
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|a Oilseeds
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|a Programmed cell death
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|a Protein processing
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|a SRA
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|a TSA
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|a VPE
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|a Vacuolar processing enzyme
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|a WGS
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|a basic local alignment search tool
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|a cDNA
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|a complementary DNA
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|a expressed sequence tag
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|a non redundant
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|a nr
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|a sequence read archive
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|a transcriptome shotgun assembly
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|a whole-genome shotgun
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|a DNA Primers
|2 NLM
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|a Oligopeptides
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Protein Sorting Signals
|2 NLM
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|a lysyl-aspartyl-glutamyl-leucine
|2 NLM
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|a 113516-56-6
|2 NLM
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| 650 |
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|a Cysteine Endopeptidases
|2 NLM
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| 650 |
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|a EC 3.4.22.-
|2 NLM
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| 650 |
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|a vacuolar processing enzyme
|2 NLM
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|a EC 3.4.22.-
|2 NLM
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|a Cysteine
|2 NLM
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|a K848JZ4886
|2 NLM
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| 700 |
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|a Soares, Emanoella L
|e verfasserin
|4 aut
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|a Costa, José H
|e verfasserin
|4 aut
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| 700 |
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|a Costa, Washington L G
|e verfasserin
|4 aut
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| 700 |
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|a Soares, Arlete A
|e verfasserin
|4 aut
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| 700 |
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|a Nogueira, Fábio C S
|e verfasserin
|4 aut
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| 700 |
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|a Domont, Gilberto B
|e verfasserin
|4 aut
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| 700 |
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|a Campos, Francisco A P
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 213(2013) vom: 15. Dez., Seite 30-7
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:213
|g year:2013
|g day:15
|g month:12
|g pages:30-7
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|u http://dx.doi.org/10.1016/j.plantsci.2013.08.009
|3 Volltext
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|a GBV_ILN_350
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|a AR
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|d 213
|j 2013
|b 15
|c 12
|h 30-7
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