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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1021/la4036858
|2 doi
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|a pubmed24n0773.xml
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|a (NLM)24152085
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Rochette, Christophe N
|e verfasserin
|4 aut
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|a Shell structure of natural rubber particles
|b evidence of chemical stratification by electrokinetics and cryo-TEM
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 11.07.2014
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|a Date Revised 26.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The interfacial structure of natural rubber (NR) colloids is investigated by means of cryogenic transmission electron microscopy (cryo-TEM) and electrokinetics over a broad range of KNO3 electrolyte concentrations (4-300 mM) and pH values (1-8). The asymptotic plateau value reached by NR electrophoretic mobility (μ) in the thin double layer limit supports the presence of a soft (ion- and water-permeable) polyelectrolytic type of layer located at the periphery of the NR particles. This property is confirmed by the analysis of the electron density profile obtained from cryo-TEM that evidences a ∼2-4 nm thick corona surrounding the NR polyisoprene core. The dependence of μ on pH and salt concentration is further marked by a dramatic decrease of the point of zero electrophoretic mobility (PZM) from 3.6 to 0.8 with increasing electrolyte concentration in the range 4-300 mM. Using a recent theory for electrohydrodynamics of soft multilayered particles, this "anomalous" dependence of the PZM on electrolyte concentration is shown to be consistent with a radial organization of anionic and cationic groups across the peripheral NR structure. The NR electrokinetic response in the pH range 1-8 is indeed found to be equivalent to that of particles surrounded by a positively charged ∼3.5 nm thick layer (mean dissociation pK ∼ 4.2) supporting a thin and negatively charged outermost layer (0.6 nm in thickness, pK ∼ 0.7). Altogether, the strong dependence of the PZM on electrolyte concentration suggests that the electrostatic properties of the outer peripheral region of the NR shell are mediated by lipidic residues protruding from a shell containing a significant amount of protein-like charges. This proposed NR shell interfacial structure questions previously reported NR representations according to which the shell consists of either a fully mixed lipid-protein layer, or a layer of phospholipids residing exclusively beneath an outer proteic film
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|a Journal Article
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|a Phospholipids
|2 NLM
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|a Proteins
|2 NLM
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|a Rubber
|2 NLM
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|a 9006-04-6
|2 NLM
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|a Crassous, Jérôme J
|e verfasserin
|4 aut
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|a Drechsler, Markus
|e verfasserin
|4 aut
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| 700 |
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|a Gaboriaud, Fabien
|e verfasserin
|4 aut
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| 700 |
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|a Eloy, Marie
|e verfasserin
|4 aut
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| 700 |
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|a de Gaudemaris, Benoît
|e verfasserin
|4 aut
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|a Duval, Jérôme F L
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 29(2013), 47 vom: 26. Nov., Seite 14655-65
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:29
|g year:2013
|g number:47
|g day:26
|g month:11
|g pages:14655-65
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|u http://dx.doi.org/10.1021/la4036858
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