|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM231752121 |
| 003 |
DE-627 |
| 005 |
20231224091454.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/jfd.12180
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0772.xml
|
| 035 |
|
|
|a (DE-627)NLM231752121
|
| 035 |
|
|
|a (NLM)24131210
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Nishiki, I
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Cloning and expression of a surface immunogenic protein in Streptococcus dysgalactiae isolated from fish and its application in enzyme-linked immunosorbent assays to diagnose S. dysgalactiae infections in fish
|
| 264 |
|
1 |
|c 2014
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 02.04.2015
|
| 500 |
|
|
|a Date Revised 30.09.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a GENBANK: AB769377
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2013 John Wiley & Sons Ltd.
|
| 520 |
|
|
|a Lancefield group C Streptococcus dysgalactiae (GCSD) causes severe necrotic lesions in the caudal peduncle in the genus Seriola farmed in Japan. To develop a sero-diagnostic method for GCSD infection in farmed fish, we attempted to identify a surface immunogenic protein that induces an antibody after infection with GCSD by immunoblot analysis using sera collected from infected fish. A protein obtained from sodium dodecyl sulfate (SDS) extracts of GCSD was identified as S. dysgalactiae surface immunogenic protein (Sd-Sip). Sd-Sip exhibited more than 94% homology with a surface antigen or a hypothetical protein from S. dysgalactiae mammalian isolates at the nucleotide sequence level. Expression of the recombinant Sd-Sip (rSd-Sip) was confirmed by immunoblot analysis, that is, its reactivity to GCSD-infected sera. Antibody detection ELISA using rSd-Sip and their usefulness for diagnosis of GCSD infection were examined. GCSD-infected sera collected from farmed amberjack, Seriola dumerili (Risso), showed strong reaction with immobilized rSd-Sip. Meanwhile, sera immunized by other pathogenic bacteria of fish were showed ELISA values similar to those of non-infected sera. These results of this study suggest that the antibody detection ELISA using rSd-Sip is an effective diagnostic method for GCSD infection in fish
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Streptococcus dysgalactiae
|
| 650 |
|
4 |
|a diagnosis
|
| 650 |
|
4 |
|a enzyme-linked immunosorbent assay
|
| 650 |
|
4 |
|a surface immunogenic protein
|
| 650 |
|
7 |
|a Antigens, Bacterial
|2 NLM
|
| 700 |
1 |
|
|a Minami, T
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Itami, T
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yoshida, T
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 37(2014), 12 vom: 04. Dez., Seite 1031-9
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
|
| 773 |
1 |
8 |
|g volume:37
|g year:2014
|g number:12
|g day:04
|g month:12
|g pages:1031-9
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/jfd.12180
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 37
|j 2014
|e 12
|b 04
|c 12
|h 1031-9
|