Differentially expressed myo-inositol monophosphatase gene (CaIMP) in chickpea (Cicer arietinum L.) encodes a lithium-sensitive phosphatase enzyme with broad substrate specificity and improves seed germination and seedling growth under abiotic stresses

myo-Inositol monophosphatase (IMP) is an essential enzyme in the myo-inositol metabolic pathway where it primarily dephosphorylates myo-inositol 1-phosphate to maintain the cellular inositol pool which is important for many metabolic and signalling pathways in plants. The stress-induced increased ac...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 64(2013), 18 vom: 18. Dez., Seite 5623-39
1. Verfasser: Saxena, Saurabh C (VerfasserIn)
Weitere Verfasser: Salvi, Prafull, Kaur, Harmeet, Verma, Pooja, Petla, Bhanu Prakash, Rao, Venkateswara, Kamble, Nitin, Majee, Manoj
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Ascorbate gluconeogenesis inositol multifunctional phosphatase seed germination stress tolerance. Inositol Phosphates mehr... Plant Proteins inositol 1-phosphate 15421-51-9 Malondialdehyde 4Y8F71G49Q Lithium 9FN79X2M3F Hydrogen Peroxide BBX060AN9V Phosphoric Monoester Hydrolases EC 3.1.3.2 myo-inositol-1 (or 4)-monophosphatase EC 3.1.3.25 Ascorbic Acid PQ6CK8PD0R
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100 1 |a Saxena, Saurabh C  |e verfasserin  |4 aut 
245 1 0 |a Differentially expressed myo-inositol monophosphatase gene (CaIMP) in chickpea (Cicer arietinum L.) encodes a lithium-sensitive phosphatase enzyme with broad substrate specificity and improves seed germination and seedling growth under abiotic stresses 
264 1 |c 2013 
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337 |a ƒaComputermedien  |b c  |2 rdamedia 
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500 |a Date Completed 26.08.2014 
500 |a Date Revised 04.11.2023 
500 |a published: Print-Electronic 
500 |a GENBANK: JX069956 
500 |a Citation Status MEDLINE 
520 |a myo-Inositol monophosphatase (IMP) is an essential enzyme in the myo-inositol metabolic pathway where it primarily dephosphorylates myo-inositol 1-phosphate to maintain the cellular inositol pool which is important for many metabolic and signalling pathways in plants. The stress-induced increased accumulation of inositol has been reported in a few plants including chickpea; however, the role and regulation of IMP is not well defined in response to stress. In this work, it has been shown that IMP activity is distributed in all organs in chickpea and was noticeably enhanced during environmental stresses. Subsequently, using degenerate oligonucleotides and RACE strategy, a full-length IMP cDNA (CaIMP) was cloned and sequenced. Biochemical study revealed that CaIMP encodes a lithium-sensitive phosphatase enzyme with broad substrate specificity, although maximum activity was observed with the myo-inositol 1-phosphate and l-galactose 1-phosphate substrates. Transcript analysis revealed that CaIMP is differentially expressed and regulated in different organs, stresses and phytohormones. Complementation analysis in Arabidopsis further confirmed the role of CaIMP in l-galactose 1-phosphate and myo-inositol 1-phosphate hydrolysis and its participation in myo-inositol and ascorbate biosynthesis. Moreover, Arabidopsis transgenic plants over-expressing CaIMP exhibited improved tolerance to stress during seed germination and seedling growth, while the VTC4/IMP loss-of-function mutants exhibited sensitivity to stress. Collectively, CaIMP links various metabolic pathways and plays an important role in improving seed germination and seedling growth, particularly under stressful environments 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Ascorbate 
650 4 |a gluconeogenesis 
650 4 |a inositol 
650 4 |a multifunctional 
650 4 |a phosphatase 
650 4 |a seed germination 
650 4 |a stress tolerance. 
650 7 |a Inositol Phosphates  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a inositol 1-phosphate  |2 NLM 
650 7 |a 15421-51-9  |2 NLM 
650 7 |a Malondialdehyde  |2 NLM 
650 7 |a 4Y8F71G49Q  |2 NLM 
650 7 |a Lithium  |2 NLM 
650 7 |a 9FN79X2M3F  |2 NLM 
650 7 |a Hydrogen Peroxide  |2 NLM 
650 7 |a BBX060AN9V  |2 NLM 
650 7 |a Phosphoric Monoester Hydrolases  |2 NLM 
650 7 |a EC 3.1.3.2  |2 NLM 
650 7 |a myo-inositol-1 (or 4)-monophosphatase  |2 NLM 
650 7 |a EC 3.1.3.25  |2 NLM 
650 7 |a Ascorbic Acid  |2 NLM 
650 7 |a PQ6CK8PD0R  |2 NLM 
700 1 |a Salvi, Prafull  |e verfasserin  |4 aut 
700 1 |a Kaur, Harmeet  |e verfasserin  |4 aut 
700 1 |a Verma, Pooja  |e verfasserin  |4 aut 
700 1 |a Petla, Bhanu Prakash  |e verfasserin  |4 aut 
700 1 |a Rao, Venkateswara  |e verfasserin  |4 aut 
700 1 |a Kamble, Nitin  |e verfasserin  |4 aut 
700 1 |a Majee, Manoj  |e verfasserin  |4 aut 
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773 1 8 |g volume:64  |g year:2013  |g number:18  |g day:18  |g month:12  |g pages:5623-39 
856 4 0 |u http://dx.doi.org/10.1093/jxb/ert336  |3 Volltext 
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