|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM231659148 |
| 003 |
DE-627 |
| 005 |
20231224091244.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2013 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2013.09.016
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0772.xml
|
| 035 |
|
|
|a (DE-627)NLM231659148
|
| 035 |
|
|
|a (NLM)24121417
|
| 035 |
|
|
|a (PII)S0981-9428(13)00346-X
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Liu, Meiling
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Regulation of flavanone 3-hydroxylase gene involved in the flavonoid biosynthesis pathway in response to UV-B radiation and drought stress in the desert plant, Reaumuria soongorica
|
| 264 |
|
1 |
|c 2013
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 29.07.2014
|
| 500 |
|
|
|a Date Revised 30.09.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2013 Elsevier Masson SAS. All rights reserved.
|
| 520 |
|
|
|a Flavonoid are known to have various functions in growth, development, reproduction, and also involved in diverse stress responses in plants. However, little is known about the roles of the key enzymes in the flavonoid biosynthetic pathway in response to environmental stress, such as UV-B radiation and drought. To understand this problem, we investigated the participation of flavanone 3-hydroxylase gene (F3H), a key enzyme in flavonoid biosynthetic pathway under UV-B radiation and drought stress in the desert plant Reaumuria soongorica. A novel cDNA sequence, named as RsF3H, was isolated from R. soongorica. The deduced amino acids showed high identities to other F3Hs. A phylogenetic analysis indicated that RsF3H appeared to be most homologous to F3H from Malus domestica (MdF3H). RsF3H protein structure contained all five conserved motifs for 2-oxoglutarate-dependent dioxygenases (2-ODDs) and an Arg-X-Ser motif, all of which were also found in other F3Hs. Quantitative real-time RT-PCR analysis showed that there was a rapid increase in gene expression of RsF3H under stress. Both UV-B radiation and drought stress induced an increase in RsF3H enzyme activity and the accumulation of the products in the flavonoid biosynthetic pathway (total flavonoid and anthocyanin). The antioxidant ability (inhibition of lipid oxidation) of total flavonoid was enhanced during this study. The results suggested that one explanation of the stress tolerance of R. soongorica may be a combination of an increase in RsF3H gene expression, RsF3H enzyme activity and the anti-oxidative ability of the metabolic end products in the flavonoid biosynthetic pathway in response to UV-B radiation and drought
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a 2-ODDs
|
| 650 |
|
4 |
|a 2-oxoglutarate-dependent dioxygenases
|
| 650 |
|
4 |
|a Drought
|
| 650 |
|
4 |
|a F3H
|
| 650 |
|
4 |
|a Flavonoid biosynthetic pathway
|
| 650 |
|
4 |
|a HPLC
|
| 650 |
|
4 |
|a MDA
|
| 650 |
|
4 |
|a ORF
|
| 650 |
|
4 |
|a RACE
|
| 650 |
|
4 |
|a ROS
|
| 650 |
|
4 |
|a Reaumuria soongorica
|
| 650 |
|
4 |
|a RsF3H
|
| 650 |
|
4 |
|a TBA
|
| 650 |
|
4 |
|a TDR
|
| 650 |
|
4 |
|a UV-B
|
| 650 |
|
4 |
|a flavanone 3-hydroxylase
|
| 650 |
|
4 |
|a high performance liquid chromatography
|
| 650 |
|
4 |
|a malonaldehyde
|
| 650 |
|
4 |
|a open reading frame
|
| 650 |
|
4 |
|a qRT-PCR
|
| 650 |
|
4 |
|a quantitative real-time RT-PCR
|
| 650 |
|
4 |
|a rapid amplification of cDNA ends
|
| 650 |
|
4 |
|a reactive oxygen species
|
| 650 |
|
4 |
|a thiobarbituric acid
|
| 650 |
|
4 |
|a time domain reflectometry
|
| 650 |
|
7 |
|a Flavanones
|2 NLM
|
| 650 |
|
7 |
|a Flavonoids
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Mixed Function Oxygenases
|2 NLM
|
| 650 |
|
7 |
|a EC 1.-
|2 NLM
|
| 650 |
|
7 |
|a flavanone 3-dioxygenase
|2 NLM
|
| 650 |
|
7 |
|a EC 1.14.11.9
|2 NLM
|
| 700 |
1 |
|
|a Li, Xinrong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Liu, Yubing
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Cao, Bo
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 73(2013) vom: 14. Dez., Seite 161-7
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
|
| 773 |
1 |
8 |
|g volume:73
|g year:2013
|g day:14
|g month:12
|g pages:161-7
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2013.09.016
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 73
|j 2013
|b 14
|c 12
|h 161-7
|