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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1107/S090904951302373X
|2 doi
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|a pubmed24n0772.xml
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|a (NLM)24121354
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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|a Higashiura, Akifumi
|e verfasserin
|4 aut
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|a High-resolution X-ray crystal structure of bovine H-protein using the high-pressure cryocooling method
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 22.05.2014
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|a Date Revised 10.11.2023
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|a published: Print-Electronic
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|a PDB: 3WDN
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|a Citation Status MEDLINE
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|a Recently, many technical improvements in macromolecular X-ray crystallography have increased the number of structures deposited in the Protein Data Bank and improved the resolution limit of protein structures. Almost all high-resolution structures have been determined using a synchrotron radiation source in conjunction with cryocooling techniques, which are required in order to minimize radiation damage. However, optimization of cryoprotectant conditions is a time-consuming and difficult step. To overcome this problem, the high-pressure cryocooling method was developed (Kim et al., 2005) and successfully applied to many protein-structure analyses. In this report, using the high-pressure cryocooling method, the X-ray crystal structure of bovine H-protein was determined at 0.86 Å resolution. Structural comparisons between high- and ambient-pressure cryocooled crystals at ultra-high resolution illustrate the versatility of this technique. This is the first ultra-high-resolution X-ray structure obtained using the high-pressure cryocooling method
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a H-protein
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|a high-pressure cryocooling method
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|a high-resolution X-ray crystallography
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|a Proteins
|2 NLM
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1 |
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|a Ohta, Kazunori
|e verfasserin
|4 aut
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1 |
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|a Masaki, Mika
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sato, Masaru
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Inaka, Koji
|e verfasserin
|4 aut
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1 |
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|a Tanaka, Hiroaki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nakagawa, Atsushi
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 20(2013), Pt 6 vom: 07. Nov., Seite 989-93
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
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| 773 |
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|g volume:20
|g year:2013
|g number:Pt 6
|g day:07
|g month:11
|g pages:989-93
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|u http://dx.doi.org/10.1107/S090904951302373X
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