Real-time investigation of protein unfolding at an air-water interface at the 1 s time scale

Protein unfolding at an air-water interface has been demonstrated such that the X-ray reflectivity can be measured with an acquisition time of 1 s using a recently developed simultaneous multiple-angle-wavelength-dispersive X-ray reflectometer. This has enabled the electron density profile of the ad...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 20(2013), Pt 6 vom: 07. Nov., Seite 980-3
1. Verfasser: Yano, Yohko F (VerfasserIn)
Weitere Verfasser: Arakawa, Etsuo, Voegeli, Wolfgang, Matsushita, Tadashi
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article Research Support, Non-U.S. Gov't X-ray reflectivity air–water interface protein unfolding time-resolved measurements Water 059QF0KO0R Muramidase EC 3.2.1.17
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520 |a Protein unfolding at an air-water interface has been demonstrated such that the X-ray reflectivity can be measured with an acquisition time of 1 s using a recently developed simultaneous multiple-angle-wavelength-dispersive X-ray reflectometer. This has enabled the electron density profile of the adsorbed protein molecules to be obtained in real time. A globular protein, lysozyme, adsorbed at the air-water interface is found to unfold into a flat shape within 1 s 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a X-ray reflectivity 
650 4 |a air–water interface 
650 4 |a protein unfolding 
650 4 |a time-resolved measurements 
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700 1 |a Arakawa, Etsuo  |e verfasserin  |4 aut 
700 1 |a Voegeli, Wolfgang  |e verfasserin  |4 aut 
700 1 |a Matsushita, Tadashi  |e verfasserin  |4 aut 
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