Populus euphratica XTH overexpression enhances salinity tolerance by the development of leaf succulence in transgenic tobacco plants

Populus euphratica XTH overexpression enhances salinity tolerance by the development of leaf succulence in transgenic tobacco plants

Populus euphratica is a salt-tolerant tree species that develops leaf succulence after a prolonged period of salinity stress. In the present study, a putative xyloglucan endotransglucosylase/hydrolase gene (PeXTH) from P. euphratica was isolated and transferred to tobacco plants. PeXTH localized exc...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 64(2013), 14 vom: 02. Nov., Seite 4225-38
1. Verfasser: Han, Yansha (VerfasserIn)
Weitere Verfasser: Wang, Wei, Sun, Jian, Ding, Mingquan, Zhao, Rui, Deng, Shurong, Wang, Feifei, Hu, Yue, Wang, Yang, Lu, Yanjun, Du, Liping, Hu, Zanmin, Diekmann, Heike, Shen, Xin, Polle, Andrea, Chen, Shaoliang
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Chlorophyll a fluorescence NaCl Populus euphratica leaf anatomy photosynthesis root length salt compartmentation water-retaining capacity mehr... xyloglucan endotransglucosylase/hydrolase gene. Chlorides Plant Proteins Water 059QF0KO0R Chlorophyll 1406-65-1 Sodium 9NEZ333N27 Chlorophyll A YF5Q9EJC8Y
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100 1 |a Han, Yansha  |e verfasserin  |4 aut 
245 1 0 |a Populus euphratica XTH overexpression enhances salinity tolerance by the development of leaf succulence in transgenic tobacco plants 
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500 |a Date Revised 23.03.2024 
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520 |a Populus euphratica is a salt-tolerant tree species that develops leaf succulence after a prolonged period of salinity stress. In the present study, a putative xyloglucan endotransglucosylase/hydrolase gene (PeXTH) from P. euphratica was isolated and transferred to tobacco plants. PeXTH localized exclusively to the endoplasmic reticulum and cell wall. Plants overexpressing PeXTH were more salt tolerant than wild-type tobacco with respect to root and leaf growth, and survival. The increased capacity for salt tolerance was due mainly to the anatomical and physiological alterations caused by PeXTH overexpression. Compared with the wild type, PeXTH-transgenic plants contained 36% higher water content per unit area and 39% higher ratio of fresh weight to dry weight, a hallmark of leaf succulence. However, the increased water storage in the leaves in PeXTH-transgenic plants was not accompanied by greater leaf thickness but was due to highly packed palisade parenchyma cells and fewer intercellular air spaces between mesophyll cells. In addition to the salt dilution effect in response to NaCl, these anatomical changes increased leaf water-retaining capacity, which lowered the increase of salt concentration in the succulent tissues and mesophyll cells. Moreover, the increased number of mesophyll cells reduced the intercellular air space, which improved carbon economy and resulted in a 47-78% greater net photosynthesis under control and salt treatments (100-150 mM NaCl). Taken together, the results indicate that PeXTH overexpression enhanced salt tolerance by the development of succulent leaves in tobacco plants without swelling 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Chlorophyll a fluorescence 
650 4 |a NaCl 
650 4 |a Populus euphratica 
650 4 |a leaf anatomy 
650 4 |a photosynthesis 
650 4 |a root length 
650 4 |a salt compartmentation 
650 4 |a water-retaining capacity 
650 4 |a xyloglucan endotransglucosylase/hydrolase gene. 
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700 1 |a Wang, Wei  |e verfasserin  |4 aut 
700 1 |a Sun, Jian  |e verfasserin  |4 aut 
700 1 |a Ding, Mingquan  |e verfasserin  |4 aut 
700 1 |a Zhao, Rui  |e verfasserin  |4 aut 
700 1 |a Deng, Shurong  |e verfasserin  |4 aut 
700 1 |a Wang, Feifei  |e verfasserin  |4 aut 
700 1 |a Hu, Yue  |e verfasserin  |4 aut 
700 1 |a Wang, Yang  |e verfasserin  |4 aut 
700 1 |a Lu, Yanjun  |e verfasserin  |4 aut 
700 1 |a Du, Liping  |e verfasserin  |4 aut 
700 1 |a Hu, Zanmin  |e verfasserin  |4 aut 
700 1 |a Diekmann, Heike  |e verfasserin  |4 aut 
700 1 |a Shen, Xin  |e verfasserin  |4 aut 
700 1 |a Polle, Andrea  |e verfasserin  |4 aut 
700 1 |a Chen, Shaoliang  |e verfasserin  |4 aut 
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