Early induction of Fe-SOD gene expression is involved in tolerance to Mn toxicity in perennial ryegrass

Early induction of Fe-SOD gene expression is involved in tolerance to Mn toxicity in perennial ryegrass

Copyright © 2013. Published by Elsevier Masson SAS.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 73(2013) vom: 14. Dez., Seite 77-82
1. Verfasser: Ribera-Fonseca, Alejandra (VerfasserIn)
Weitere Verfasser: Inostroza-Blancheteau, Claudio, Cartes, Paula, Rengel, Zed, Mora, M L
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Cu/Zn-SOD Fe-SOD Gene expression Manganese tolerance Manganese toxicity Mn-SOD Oxidative stress Ryegrass mehr... Superoxide dismutase isoforms copper/zinc superoxide dismutase iron superoxide dismutase manganese superoxide dismutase qRT-PCR quantitative real-time polymerase chain reaction Plant Proteins Protein Isoforms Manganese 42Z2K6ZL8P Superoxide Dismutase EC 1.15.1.1
Beschreibung
Zusammenfassung:Copyright © 2013. Published by Elsevier Masson SAS.
Manganese (Mn) toxicity limits plant growth in acid soils. Although Mn toxicity induces oxidative stress, the role of superoxide dismutase (SOD, EC.1.15.1.1) isoforms in conferring Mn tolerance remains unclear. Seedlings of ryegrass cultivars Nui (Mn-sensitive) and Kingston (Mn-tolerant) were hydroponically grown at 2.4 (optimal) or 750 μM Mn (toxic) concentration, and harvested from 2 to 48 h. Kingston showed higher shoot Mn than Nui at 2.4 μM Mn. At toxic supply, shoot Mn concentration steadily increased in both cultivars, with Kingston having the highest accumulation at 48 h. An early (2 h) increase in lipid peroxidation under Mn excess occurred, but it returned (after 6 h) to the basal level in Kingston only. Kingston exhibited higher SOD activity than Nui, and that difference increased due to toxic Mn. In general, Mn-induced gene expression of Mn- and Cu/Zn-SOD isoforms was higher in Nui than Kingston. Nevertheless, under Mn excess, we found a greater Fe-SOD up-regulation (up to 5-fold) in Kingston compared to Nui. Thus, Fe-SOD induction in Kingston might explain, at least partly, its high tolerance to Mn toxicity. This is the first evidence that Mn toxicity causes differential gene expression of SOD isoforms in ryegrass cultivars in the short-term
Beschreibung:Date Completed 29.07.2014
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2013.08.012