Using DNA-driven assembled phospholipid nanodiscs as a scaffold for gold nanoparticle patterning
Recently, a new class of materials emerged with the assembly of DNA-coated phospholipid nanodiscs into columnar BioNanoStacks. Within these stacks, lipid discs are periodically incorporated, resulting into quasi-one-dimensional superstructures. With each disc surrounded by two recombinant scaffoldin...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 29(2013), 42 vom: 22. Okt., Seite 13089-94 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, Non-P.H.S. Membrane Proteins Phospholipids Gold 7440-57-5 Nickel 7OV03QG267 DNA 9007-49-2 |
| Zusammenfassung: | Recently, a new class of materials emerged with the assembly of DNA-coated phospholipid nanodiscs into columnar BioNanoStacks. Within these stacks, lipid discs are periodically incorporated, resulting into quasi-one-dimensional superstructures. With each disc surrounded by two recombinant scaffolding proteins, we decided to examine whether the polyhistidine tags of these proteins could be utilized to bind additional molecules or particles to these BioNanoStacks. Here we demonstrate that patterning of gold nanoparticles onto these BioNanoStacks is indeed possible. Binding occurs via a nickel-mediated interaction between the nanogolds nitrilotriacetic acid and the histidine tags of the scaffold proteins surrounding the nanodiscs. Using Monte Carlo simulations, we determine that the binding of the nanogold particles to the stacks is not a random event. By comparing the simulation and experimental results, we find that there are preferred binding sites, which affects the binding statistics |
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| Beschreibung: | Date Completed 06.06.2014 Date Revised 22.10.2013 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la403091w |