Structural and functional characterization of annexin 1 from Medicago truncatula

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 73(2013) vom: 05. Dez., Seite 56-62
1. Verfasser: Kodavali, Praveen Kumar (VerfasserIn)
Weitere Verfasser: Skowronek, Krzysztof, Koszela-Piotrowska, Izabela, Strzelecka-Kiliszek, Agnieszka, Pawlowski, Krzysztof, Pikula, Slawomir
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 3-D AnnAt1–AnnAt8 AnnMt1–AnnMt9 Annexin 1 AnxA1–A11 and AnxA13 CVs Ion channel Medicago truncatula mehr... PMSF Protein structure annexins 1–8 from Arabidopsis thaliana annexins 1–9 from Medicago truncatula column volumes mammalian annexins A1–A11 and A13 phenyl methyl sulfonyl fluoride three-dimensional Annexins Cations Ion Channels Plant Proteins Recombinant Proteins
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520 |a Annexins are calcium- and membrane-binding proteins that have been shown to have diverse properties such as actin, integrin and GTP binding, both in animals and plants. Recently, Medicago truncatula annexin 1 (AnnMt1) has been suggested to participate in nodulation (Nod factor signaling) and mycorrhization in legume plants. In this report we demonstrate for the first time that recombinant AnnMt1 (rec-AnnMt1) mediates membrane permeabilization to cations with conductance ranging from 16 pS to 329 pS. In agreement with other structurally determined annexins, homology modeling of AnnMt1 suggests that most of the functional determinants are found on the convex surface of the modeled structure. In conclusion, we propose a potential constitutive role of AnnMt1 in Nod factor signaling as a non-specific ion channel 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a 3-D 
650 4 |a AnnAt1–AnnAt8 
650 4 |a AnnMt1–AnnMt9 
650 4 |a Annexin 1 
650 4 |a AnxA1–A11 and AnxA13 
650 4 |a CVs 
650 4 |a Ion channel 
650 4 |a Medicago truncatula 
650 4 |a PMSF 
650 4 |a Protein structure 
650 4 |a annexins 1–8 from Arabidopsis thaliana 
650 4 |a annexins 1–9 from Medicago truncatula 
650 4 |a column volumes 
650 4 |a mammalian annexins A1–A11 and A13 
650 4 |a phenyl methyl sulfonyl fluoride 
650 4 |a three-dimensional 
650 7 |a Annexins  |2 NLM 
650 7 |a Cations  |2 NLM 
650 7 |a Ion Channels  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Recombinant Proteins  |2 NLM 
700 1 |a Skowronek, Krzysztof  |e verfasserin  |4 aut 
700 1 |a Koszela-Piotrowska, Izabela  |e verfasserin  |4 aut 
700 1 |a Strzelecka-Kiliszek, Agnieszka  |e verfasserin  |4 aut 
700 1 |a Pawlowski, Krzysztof  |e verfasserin  |4 aut 
700 1 |a Pikula, Slawomir  |e verfasserin  |4 aut 
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773 1 8 |g volume:73  |g year:2013  |g day:05  |g month:12  |g pages:56-62 
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