Molecular cloning and expression analysis of PDR1-like gene in ginseng subjected to salt and cold stresses or hormonal treatment

Molecular cloning and expression analysis of PDR1-like gene in ginseng subjected to salt and cold stresses or hormonal treatment

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 71(2013) vom: 19. Okt., Seite 203-11
1. Verfasser: Zhang, Ru (VerfasserIn)
Weitere Verfasser: Zhu, Jie, Cao, Hong-Zhe, An, Yan-Ru, Huang, Jing-Jia, Chen, Xiang-Hui, Mohammed, Nuruzzaman, Afrin, Sadia, Luo, Zhi-Yong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ABA ABC ATP-binding cassette CaMV DIG Environmental stresses GFP Gene expression mehr... Hormones JA MDR MDR-associated protein MRP MS MeJA Murashige and Skoog NBD ORF PDR Panax ginseng Pleiotropic drug resistance transporter RACE SA SAR TMD abscisic acid cauliflower mosaic virus digoxigenin green fluorescent protein jasmonic acid methyl jasmonate multidrug resistance nucleotide binding domain open reading frame pleiotropic drug resistance qRT-RCR quantitative real-time PCR rapid-amplification of cDNA end salicylic acid systemic acquired resistance transmembrane domain Plant Proteins Salts
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245 1 0 |a Molecular cloning and expression analysis of PDR1-like gene in ginseng subjected to salt and cold stresses or hormonal treatment 
264 1 |c 2013 
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500 |a Date Completed 02.05.2014 
500 |a Date Revised 30.09.2020 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Copyright © 2013 Elsevier Masson SAS. All rights reserved. 
520 |a The plant pleiotropic drug resistance (PDR) family of ATP-binding cassette (ABC) transporters is potentially involved in diverse biological processes. Currently, little is known about their actual physiological functions. A Panax ginseng PDR transporter gene (PgPDR1) was cloned and the cDNA has an open reading frame of 4344 bp. The deduced amino acid sequence contained the characteristic domains of PDR transporters: Walker A, Walker B, and ABC signature. Genomic DNA hybridization analysis indicated that one copy of PgPDR1 gene was present in P. ginseng. Subcellular localization showed that PgPDR1-GFP fusion protein was specifically localized in the cell membrane. Promoter region analysis revealed the presence of cis-acting elements, some of which are putatively involved in response to hormone, light and stress. To understand the functional roles of PgPDR1, we investigated the expression patterns of PgPDR1 in different tissues and under various conditions. Quantitative real-time PCR (qRT-PCR) and Western blotting analysis showed that PgPDR1 was expressed at a high level in the roots and leaves compared to seeds and stems. The expression of PgPDR1 was up-regulated by salicylic acid (SA) or chilling, down-regulated by ABA, and regulated differently at transcript and protein levels by MeJA. These results suggest that PgPDR1 might be involved in responding to environmental stresses and hormones 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a ABA 
650 4 |a ABC 
650 4 |a ATP-binding cassette 
650 4 |a CaMV 
650 4 |a DIG 
650 4 |a Environmental stresses 
650 4 |a GFP 
650 4 |a Gene expression 
650 4 |a Hormones 
650 4 |a JA 
650 4 |a MDR 
650 4 |a MDR-associated protein 
650 4 |a MRP 
650 4 |a MS 
650 4 |a MeJA 
650 4 |a Murashige and Skoog 
650 4 |a NBD 
650 4 |a ORF 
650 4 |a PDR 
650 4 |a Panax ginseng 
650 4 |a Pleiotropic drug resistance transporter 
650 4 |a RACE 
650 4 |a SA 
650 4 |a SAR 
650 4 |a TMD 
650 4 |a abscisic acid 
650 4 |a cauliflower mosaic virus 
650 4 |a digoxigenin 
650 4 |a green fluorescent protein 
650 4 |a jasmonic acid 
650 4 |a methyl jasmonate 
650 4 |a multidrug resistance 
650 4 |a nucleotide binding domain 
650 4 |a open reading frame 
650 4 |a pleiotropic drug resistance 
650 4 |a qRT-RCR 
650 4 |a quantitative real-time PCR 
650 4 |a rapid-amplification of cDNA end 
650 4 |a salicylic acid 
650 4 |a systemic acquired resistance 
650 4 |a transmembrane domain 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Salts  |2 NLM 
700 1 |a Zhu, Jie  |e verfasserin  |4 aut 
700 1 |a Cao, Hong-Zhe  |e verfasserin  |4 aut 
700 1 |a An, Yan-Ru  |e verfasserin  |4 aut 
700 1 |a Huang, Jing-Jia  |e verfasserin  |4 aut 
700 1 |a Chen, Xiang-Hui  |e verfasserin  |4 aut 
700 1 |a Mohammed, Nuruzzaman  |e verfasserin  |4 aut 
700 1 |a Afrin, Sadia  |e verfasserin  |4 aut 
700 1 |a Luo, Zhi-Yong  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 71(2013) vom: 19. Okt., Seite 203-11  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:71  |g year:2013  |g day:19  |g month:10  |g pages:203-11 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2013.07.011  |3 Volltext 
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