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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1021/la401566h
|2 doi
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|a pubmed24n0766.xml
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|a DE-627
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|a eng
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| 100 |
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|a Koeda, Shuhei
|e verfasserin
|4 aut
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| 245 |
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|a Creation of cross-linked bilayer membranes that can incorporate membrane proteins from oligo-Asp-based peptide gemini surfactants
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 04.04.2014
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|a Date Revised 17.09.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We designed novel bilayer-forming amphiphiles based on the cyclic oligo-Asp-based peptide gemini (PG) surfactants cr-D2C12 and cr-D3C12, which consist of -Cys(Asp)nCys- (n = 2 or 3) as a core peptide and two Cys residues containing a dodecylamidomethyl group. Dynamic light scattering and transmission electron microscopy measurements revealed the formation of spherical bilayer membranes that could incorporate the light-harvesting antenna complex 2 (LH2) from Rhodopseudomonas acidophila . Furthermore, this proteoliposome-like conjugate could be assembled onto cationized glass and mica to form planar bilayer membranes incorporating LH2. Using atomic force microscopy, we observed LH2 protruding (ca. 1.2-1.5 nm) from flat terraces of the planar bilayer membranes formed from cr-D2C12 or cr-D3C12. Thus, our designed PG surfactants are a new class of bilayer-forming amphiphiles that may be applied to the study of various membrane proteins
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cross-Linking Reagents
|2 NLM
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|a Light-Harvesting Protein Complexes
|2 NLM
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|a Lipid Bilayers
|2 NLM
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|a Peptides
|2 NLM
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|a Surface-Active Agents
|2 NLM
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|a Aspartic Acid
|2 NLM
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|a 30KYC7MIAI
|2 NLM
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|a Umezaki, Katsunari
|e verfasserin
|4 aut
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|a Sumino, Ayumi
|e verfasserin
|4 aut
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|a Noji, Tomoyasu
|e verfasserin
|4 aut
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|a Ikeda, Atsushi
|e verfasserin
|4 aut
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|a Yamamoto, Yasushi
|e verfasserin
|4 aut
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| 700 |
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|a Dewa, Takehisa
|e verfasserin
|4 aut
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| 700 |
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|a Taga, Keijiro
|e verfasserin
|4 aut
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| 700 |
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|a Nango, Mamoru
|e verfasserin
|4 aut
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|a Tanaka, Toshiki
|e verfasserin
|4 aut
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|a Mizuno, Toshihisa
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 29(2013), 37 vom: 17. Sept., Seite 11695-704
|w (DE-627)NLM098181009
|x 1520-5827
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| 773 |
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|g volume:29
|g year:2013
|g number:37
|g day:17
|g month:09
|g pages:11695-704
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|u http://dx.doi.org/10.1021/la401566h
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