A type III ACC synthase, ACS7, is involved in root gravitropism in Arabidopsis thaliana

Ethylene is an important plant hormone that regulates developmental processes in plants. The ethylene biosynthesis pathway is a highly regulated process at both the transcriptional and post-translational level. The transcriptional regulation of these ethylene biosynthesis genes is well known. Howeve...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 64(2013), 14 vom: 10. Nov., Seite 4343-60
1. Verfasser: Huang, Shih-Jhe (VerfasserIn)
Weitere Verfasser: Chang, Chia-Lun, Wang, Po-Hsun, Tsai, Min-Chieh, Hsu, Pang-Hung, Chang, Ing-Feng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 14-3-3 ACS CDPK calcium ethylene phosphorylation root gravitropism. 14-3-3 Proteins mehr... Amino Acids, Cyclic Chelating Agents Ethylenes Peptides Small Ubiquitin-Related Modifier Proteins 1-aminocyclopropane-1-carboxylic acid 3K9EJ633GL 91GW059KN7 Protein Kinases EC 2.7.- calcium-dependent protein kinase EC 2.7.1.- Lyases EC 4.- 1-aminocyclopropanecarboxylate synthase EC 4.4.1.14 Lithium Chloride G4962QA067 Calcium SY7Q814VUP
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245 1 2 |a A type III ACC synthase, ACS7, is involved in root gravitropism in Arabidopsis thaliana 
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500 |a Date Revised 21.10.2021 
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520 |a Ethylene is an important plant hormone that regulates developmental processes in plants. The ethylene biosynthesis pathway is a highly regulated process at both the transcriptional and post-translational level. The transcriptional regulation of these ethylene biosynthesis genes is well known. However, post-translational modifications of the key ethylene biosynthesis enzyme 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) are little understood. In vitro kinase assays were conducted on the type III ACS, AtACS7, fusion protein and peptides to determine whether the AtACS7 protein can be phosphorylated by calcium-dependent protein kinase (CDPK). AtACS7 was phosphorylated at Ser216, Thr296, and Ser299 by AtCDPK16 in vitro. To investigate further the function of the ACS7 gene in Arabidopsis, an acs7-1 loss-of-function mutant was isolated. The acs7-1 mutant exhibited less sensitivity to the inhibition of root gravitropism by treatment with the calcium chelator ethylene glycol tetraacetic acid (EGTA). Seedlings were treated with gradient concentrations of ACC. The results showed that a certain concentration of ethylene enhanced the gravity response. Moreover, the acs7-1 mutant was less sensitive to inhibition of the gravity response by treatment with the auxin polar transport inhibitor 1-naphthylphthalamic acid, but exogenous ACC application recovered root gravitropism. Altogether, the results indicate that AtACS7 is involved in root gravitropism in a calcium-dependent manner in Arabidopsis 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a 14-3-3 
650 4 |a ACS 
650 4 |a CDPK 
650 4 |a calcium 
650 4 |a ethylene 
650 4 |a phosphorylation 
650 4 |a root gravitropism. 
650 7 |a 14-3-3 Proteins  |2 NLM 
650 7 |a Amino Acids, Cyclic  |2 NLM 
650 7 |a Chelating Agents  |2 NLM 
650 7 |a Ethylenes  |2 NLM 
650 7 |a Peptides  |2 NLM 
650 7 |a Small Ubiquitin-Related Modifier Proteins  |2 NLM 
650 7 |a 1-aminocyclopropane-1-carboxylic acid  |2 NLM 
650 7 |a 3K9EJ633GL  |2 NLM 
650 7 |a ethylene  |2 NLM 
650 7 |a 91GW059KN7  |2 NLM 
650 7 |a Protein Kinases  |2 NLM 
650 7 |a EC 2.7.-  |2 NLM 
650 7 |a calcium-dependent protein kinase  |2 NLM 
650 7 |a EC 2.7.1.-  |2 NLM 
650 7 |a Lyases  |2 NLM 
650 7 |a EC 4.-  |2 NLM 
650 7 |a 1-aminocyclopropanecarboxylate synthase  |2 NLM 
650 7 |a EC 4.4.1.14  |2 NLM 
650 7 |a Lithium Chloride  |2 NLM 
650 7 |a G4962QA067  |2 NLM 
650 7 |a Calcium  |2 NLM 
650 7 |a SY7Q814VUP  |2 NLM 
700 1 |a Chang, Chia-Lun  |e verfasserin  |4 aut 
700 1 |a Wang, Po-Hsun  |e verfasserin  |4 aut 
700 1 |a Tsai, Min-Chieh  |e verfasserin  |4 aut 
700 1 |a Hsu, Pang-Hung  |e verfasserin  |4 aut 
700 1 |a Chang, Ing-Feng  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of experimental botany  |d 1985  |g 64(2013), 14 vom: 10. Nov., Seite 4343-60  |w (DE-627)NLM098182706  |x 1460-2431  |7 nnns 
773 1 8 |g volume:64  |g year:2013  |g number:14  |g day:10  |g month:11  |g pages:4343-60 
856 4 0 |u http://dx.doi.org/10.1093/jxb/ert241  |3 Volltext 
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