A comprehensive genetic study reveals a crucial role of CYP90D2/D2 in regulating plant architecture in rice (Oryza sativa)

© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 200(2013), 4 vom: 19. Dez., Seite 1076-88
1. Verfasser: Li, Hui (VerfasserIn)
Weitere Verfasser: Jiang, Ling, Youn, Ji-Hyun, Sun, Wei, Cheng, Zhijun, Jin, Tianyun, Ma, Xiaoding, Guo, Xiuping, Wang, Jiulin, Zhang, Xin, Wu, Fuqing, Wu, Chuanyin, Kim, Seong-Ki, Wan, Jianmin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't C-23 hydroxylase C-3 dehydrogenase CYP90D2/D2 brassinosteroid biosynthesis chromosome segment deleted dwarf 1 (csdd1) dwarf plant architecture rice (Oryza sativa) mehr... Brassinosteroids Plant Proteins
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520 |a Brassinosteroids (BRs) are essential regulators of plant architecture. Understanding how BRs control plant height and leaf angle would facilitate development of new plant type varieties by biotechnology. A number of mutants involved in BR biosynthesis have been isolated but many of them lack detailed genetic analysis. Here, we report the isolation and characterization of a severe dwarf mutant, chromosome segment deleted dwarf 1 (csdd1), which was deficient in BR biosynthesis in rice. We isolated the mutant by screening a tissue culture-derived population, cloned the gene by mapping, and confirmed its function by complementary and RNAi experiments, combined with physiological and chemical analysis. We showed that the severe dwarf phenotype was caused by a complete deletion of a cytochrome P450 gene, CYP90D2/D2, which was further confirmed in two independent T-DNA insertion lines in different genetic backgrounds and by RNA interference. Our chemical analysis suggested that CYP90D2/D2 might catalyze C-3 dehydrogenation step in BR biosynthesis. We have demonstrated that the CYP90D2/D2 gene plays a more important role than previously reported. Allelic mutations of CYP90D2/D2 confer varying degrees of dwarfism and leaf angle, thus providing useful information for molecular breeding in grain crop plants 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 4 |a CYP90D2/D2 
650 4 |a brassinosteroid biosynthesis 
650 4 |a chromosome segment deleted dwarf 1 (csdd1) 
650 4 |a dwarf 
650 4 |a plant architecture 
650 4 |a rice (Oryza sativa) 
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650 7 |a Plant Proteins  |2 NLM 
700 1 |a Jiang, Ling  |e verfasserin  |4 aut 
700 1 |a Youn, Ji-Hyun  |e verfasserin  |4 aut 
700 1 |a Sun, Wei  |e verfasserin  |4 aut 
700 1 |a Cheng, Zhijun  |e verfasserin  |4 aut 
700 1 |a Jin, Tianyun  |e verfasserin  |4 aut 
700 1 |a Ma, Xiaoding  |e verfasserin  |4 aut 
700 1 |a Guo, Xiuping  |e verfasserin  |4 aut 
700 1 |a Wang, Jiulin  |e verfasserin  |4 aut 
700 1 |a Zhang, Xin  |e verfasserin  |4 aut 
700 1 |a Wu, Fuqing  |e verfasserin  |4 aut 
700 1 |a Wu, Chuanyin  |e verfasserin  |4 aut 
700 1 |a Kim, Seong-Ki  |e verfasserin  |4 aut 
700 1 |a Wan, Jianmin  |e verfasserin  |4 aut 
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