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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1177/0734242X13492841
|2 doi
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|a pubmed24n0764.xml
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|a DE-627
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|a eng
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|a Escamilla-Alvarado, Carlos
|e verfasserin
|4 aut
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|a Use of organic waste for the production of added-value holocellulases with Cellulomonas flavigena PR-22 and Trichoderma reesei MCG 80
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|c 2013
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 06.02.2014
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|a Date Revised 21.09.2015
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We evaluated the production of holocellulases from the cellulolytic microorganisms Cellulomonas flavigena PR-22 and Trichoderma reesei MCG 80 using as substrates the organic fraction of municipal solid waste (OFMSW) and digestates from a hydrogenogenic-methanogenic bioenergy production process. The first set of experiments (E1) used the mutant actinobacteria C. flavigena PR-22 whereas another set (E2) used the mutant filamentous fungi T. reesei MCG 80. In E1 with OFMSW as substrate, xylanolytic activities ranged from 1800 to 3900 international units g(holocellulose)(-1) (IU g(hol)(-1)), whereas the cellulolytic activities ranged from 220 to 420 IU g(hol)(-1). The variation of agitation speed did not have a significant effect on enzyme activity, whereas the increase of substrate concentration had a significant negative effect on both xylanolytic and cellulolytic activities on a holocellulose feed basis. Regarding E2, the OFMSW was evaluated at 1, 2 and 3 % volatile solids (VS). At 2 % VS the best filter paper activities were 1200 filter paper units (FPU) l(-1); however, in a holocellulase basis the best result was 67 FPU g(hol)(-1), corresponding to 1 % VS. Next, OFMSW was compared with OFMSW supplemented with lactose, digested solids from hydrogenogenic fermentation (D1) and digested solids from a two-stage process (D2). Against expectations, no positive effect was found in OFMSW due to lactose. The best enzymatic titres were in the order D1 > OFMSW ≈ OFMSW + lactose > D2. The use of digestates from hydrogenogenic fermentation for enzyme production holds promise for waste management. It promotes energy and added-value bioproduct generation-a green alternative to common practice of management and disposal of organic wastes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Biorefinery
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|a Cellulomonas flavigena
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|a Trichoderma reesei
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|a cellulases
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|a digestates
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|a organic waste
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|a xylanases
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|a Cellulase
|2 NLM
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| 650 |
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|a EC 3.2.1.4
|2 NLM
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|a Poggi-Varaldo, Héctor M
|e verfasserin
|4 aut
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|a Ponce-Noyola, M Teresa
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA
|d 1991
|g 31(2013), 8 vom: 01. Aug., Seite 849-58
|w (DE-627)NLM098164791
|x 1096-3669
|7 nnns
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| 773 |
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|g volume:31
|g year:2013
|g number:8
|g day:01
|g month:08
|g pages:849-58
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|u http://dx.doi.org/10.1177/0734242X13492841
|3 Volltext
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