Identification of potential miRNAs and their targets in Vriesea carinata (Poales, Bromeliaceae)
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
| Veröffentlicht in: | Plant science : an international journal of experimental plant biology. - 1985. - 210(2013) vom: 04. Sept., Seite 214-23 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | Plant science : an international journal of experimental plant biology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't High-throughput sequencing MFEI Stress response Vriesea carinata miRNA miRNAs microRNA microRNA precursor mehr... |
| Zusammenfassung: | Copyright © 2013 Elsevier Ireland Ltd. All rights reserved. The miRNAs play important roles in regulation of gene expression at the post-transcriptional level. A small RNA and RNA-seq of libraries were constructed to identify miRNAs in Vriesea carinata, a native bromeliad species from Brazilian Atlantic Rainforest. Illumina technology was used to perform high throughput sequencing and data was analyzed using bioinformatics tools. We obtained 2,191,509 mature miRNAs sequences representing 54 conserved families in plant species. Further analysis allowed the prediction of secondary structures for 19 conserved and 16 novel miRNAs. Potential targets were predicted from pre-miRNAs by sequence homology and validated using RTqPCR approach. This study provides the first identification of miRNAs and their potential targets of a bromeliad species |
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| Beschreibung: | Date Completed 27.01.2014 Date Revised 30.03.2022 published: Print-Electronic GEO: GSE38250 Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2013.05.013 |