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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1111/nph.12405
|2 doi
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|a pubmed24n1342.xml
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|a (DE-627)NLM229078567
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|a (NLM)23845035
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Jones, David L
|e verfasserin
|4 aut
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| 245 |
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|a Competition between plant and bacterial cells at the microscale regulates the dynamics of nitrogen acquisition in wheat (Triticum aestivum)
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 29.04.2014
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|a Date Revised 23.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
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|a The ability of plants to compete effectively for nitrogen (N) resources is critical to plant survival. However, controversy surrounds the importance of organic and inorganic sources of N in plant nutrition because of our poor ability to visualize and understand processes happening at the root-microbial-soil interface. Using high-resolution nano-scale secondary ion mass spectrometry stable isotope imaging (NanoSIMS-SII), we quantified the fate of ¹⁵N over both space and time within the rhizosphere. We pulse-labelled the soil surrounding wheat (Triticum aestivum) roots with either ¹⁵NH₄⁺ or ¹⁵N-glutamate and traced the movement of ¹⁵N over 24 h. Imaging revealed that glutamate was rapidly depleted from the rhizosphere and that most ¹⁵N was captured by rhizobacteria, leading to very high ¹⁵N microbial enrichment. After microbial capture, approximately half of the ¹⁵N-glutamate was rapidly mineralized, leading to the excretion of NH₄⁺, which became available for plant capture. Roots proved to be poor competitors for ¹⁵N-glutamate and took up N mainly as ¹⁵NH₄⁺. Spatial mapping of ¹⁵N revealed differential patterns of ¹⁵N uptake within bacteria and the rapid uptake and redistribution of ¹⁵N within roots. In conclusion, we demonstrate the rapid cycling and transformation of N at the soil-root interface and that wheat capture of organic N is low in comparison to inorganic N under the conditions tested
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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|a NanoSIMS
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|a amino acids
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|a dissolved organic nitrogen
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|a nitrogen cycling
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|a nutrient uptake
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|a rhizobacteria
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|a rhizosphere architecture
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|a Ammonium Compounds
|2 NLM
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| 650 |
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|a Nitrogen Isotopes
|2 NLM
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| 650 |
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|a Soil
|2 NLM
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|a Glutamic Acid
|2 NLM
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| 650 |
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|a 3KX376GY7L
|2 NLM
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| 650 |
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|a Nitrogen
|2 NLM
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| 650 |
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|a N762921K75
|2 NLM
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| 700 |
1 |
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|a Clode, Peta L
|e verfasserin
|4 aut
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1 |
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|a Kilburn, Matt R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Stockdale, Elizabeth A
|e verfasserin
|4 aut
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| 700 |
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|a Murphy, Daniel V
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 200(2013), 3 vom: 10. Nov., Seite 796-807
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:200
|g year:2013
|g number:3
|g day:10
|g month:11
|g pages:796-807
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|u http://dx.doi.org/10.1111/nph.12405
|3 Volltext
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