Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b overexpression enhances water productivity, resistance to drought, and infection

Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b overexpression enhances water productivity, resistance to drought, and infection

Heat-stressed crops suffer dehydration, depressed growth, and a consequent decline in water productivity, which is the yield of harvestable product as a function of lifetime water consumption and is a trait associated with plant growth and development. Heat shock transcription factor (HSF) genes hav...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 64(2013), 11 vom: 02. Aug., Seite 3467-81
1. Verfasser: Bechtold, Ulrike (VerfasserIn)
Weitere Verfasser: Albihlal, Waleed S, Lawson, Tracy, Fryer, Michael J, Sparrow, Penelope A C, Richard, François, Persad, Ramona, Bowden, Laura, Hickman, Richard, Martin, Cathie, Beynon, Jim L, Buchanan-Wollaston, Vicky, Baker, Neil R, Morison, James I L, Schöffl, Friedrich, Ott, Sascha, Mullineaux, Philip M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana Brassica napus Hyaloperonospora parasitica Pseudomonas syringae basal resistance biotic and abiotic stress drought stress heat stress mehr... hydrogen peroxide transcription factors water productivity. Arabidopsis Proteins DNA-Binding Proteins Heat Shock Transcription Factors Transcription Factors Water 059QF0KO0R
Beschreibung
Zusammenfassung:Heat-stressed crops suffer dehydration, depressed growth, and a consequent decline in water productivity, which is the yield of harvestable product as a function of lifetime water consumption and is a trait associated with plant growth and development. Heat shock transcription factor (HSF) genes have been implicated not only in thermotolerance but also in plant growth and development, and therefore could influence water productivity. Here it is demonstrated that Arabidopsis thaliana plants with increased HSFA1b expression showed increased water productivity and harvest index under water-replete and water-limiting conditions. In non-stressed HSFA1b-overexpressing (HSFA1bOx) plants, 509 genes showed altered expression, and these genes were not over-represented for development-associated genes but were for response to biotic stress. This confirmed an additional role for HSFA1b in maintaining basal disease resistance, which was stress hormone independent but involved H₂O₂ signalling. Fifty-five of the 509 genes harbour a variant of the heat shock element (HSE) in their promoters, here named HSE1b. Chromatin immunoprecipitation-PCR confirmed binding of HSFA1b to HSE1b in vivo, including in seven transcription factor genes. One of these is MULTIPROTEIN BRIDGING FACTOR1c (MBF1c). Plants overexpressing MBF1c showed enhanced basal resistance but not water productivity, thus partially phenocopying HSFA1bOx plants. A comparison of genes responsive to HSFA1b and MBF1c overexpression revealed a common group, none of which harbours a HSE1b motif. From this example, it is suggested that HSFA1b directly regulates 55 HSE1b-containing genes, which control the remaining 454 genes, collectively accounting for the stress defence and developmental phenotypes of HSFA1bOx
Beschreibung:Date Completed 22.10.2013
Date Revised 17.03.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/ert185