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|a eng
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| 100 |
1 |
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|a Chen, Minghan
|e verfasserin
|4 aut
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| 245 |
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|a Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|2 rdacarrier
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|a Date Revised 21.10.2021
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a We report the use of total internal reflection fluorescence (TIRF) microscopy for analyzing receptor pharmacology and the development of a microplate-compatible TIRF imaging system. Using stably expressed green fluorescence protein tagged β2-adrenergic receptor as the reporter, we found that the activation of different receptors results in distinct kinetic signatures of the TIRF intensity of cells. These TIRF signatures closely resemble the characteristics of their respective label-free dynamic mass redistribution signals in the same cells. This suggests that TIRF in microplate can be used for profiling and screening drugs
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|a Journal Article
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|a Zaytseva, Natalya V
|e verfasserin
|4 aut
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| 700 |
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|a Wu, Qi
|e verfasserin
|4 aut
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| 700 |
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|a Li, Min
|e verfasserin
|4 aut
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| 700 |
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|a Fang, Ye
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Applied physics letters
|d 1998
|g 102(2013), 19 vom: 13. Mai, Seite 193702
|w (DE-627)NLM098165984
|x 0003-6951
|7 nnns
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| 773 |
1 |
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|g volume:102
|g year:2013
|g number:19
|g day:13
|g month:05
|g pages:193702
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