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|a eng
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|a Gao, Liang
|e verfasserin
|4 aut
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|a Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry
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|c 2013
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|a Text
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|a ohne Hilfsmittel zu benutzen
|b n
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|a Date Revised 21.10.2021
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here, we present a technique-fluorescence-assisted photoacoustic thermometry (FAPT)-for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 °C) temperature resolution and sub-micron (0.23 μm) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration- and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing
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|a Journal Article
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|a Zhang, Chi
|e verfasserin
|4 aut
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|a Li, Chiye
|e verfasserin
|4 aut
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|a Wang, Lihong V
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Applied physics letters
|d 1998
|g 102(2013), 19 vom: 13. Mai, Seite 193705
|w (DE-627)NLM098165984
|x 0003-6951
|7 nnns
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| 773 |
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|g volume:102
|g year:2013
|g number:19
|g day:13
|g month:05
|g pages:193705
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