Expression profiling of genes involved in ascorbate biosynthesis and recycling during fleshy root development in radish

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 70(2013) vom: 14. Sept., Seite 269-77
1. Verfasser:	Xu, Yao (VerfasserIn)
Weitere Verfasser:	Zhu, Xianwen, Chen, Yinglong, Gong, Yiqin, Liu, Liwang
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't 4,7-diphenyl-1,10-phenanthroline AO APX AsA Ascorbate BT DHAR EtOH mehr... GDP-l-galactose-1-phosphate phosphorylase GDP-mannose pyrophosphorylase GDP-mannose-3′,5′-epimerase GGP GLDH GME GMP GPP GR GalDH GalUR Gene expression MDHAR MIOX PGI PMI PMM R/S ROS RT-PCR Raphanus sativus L. Root development T-AsA TCA ascorbate ascorbate oxidase ascorbate peroxidase d-galacturonate reductase dehydroascorbate reductase ethyl alcohol glutathione reductase l-galactono-1,4-lactone dehydrogenase l-galactose dehydrogenase l-galactose pathway l-galactose-1-phosphate phosphatase monodehydroascorbate reductase myo-inositol oxygenase phosphoglucose isomerase phosphomannose isomerase phosphomannose mutase reactive oxygen species reverse transcription and polymerase chain reaction root–shoot ratio total ascorbate trichloroacetic acid Antioxidants Plant Proteins Alcohol Oxidoreductases EC 1.1.- Galactose Dehydrogenases EC 1.1.1.- NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases D-galacturonate reductase EC 1.1.1.365 galactose dehydrogenase EC 1.1.1.48 Phosphorylases EC 2.4.1.- Ascorbic Acid PQ6CK8PD0R Galactose X2RN3Q8DNE

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier Masson SAS. All rights reserved. Ascorbate is a primary antioxidant and an essential enzyme cofactor in plants, which has an important effect on the development of plant root system. To investigate the molecular mechanisms of ascorbate accumulation during root development and reveal the key genes of the ascorbate biosynthesis and recycling pathways, the expression of 16 related genes together with ascorbate abundance were analyzed in the flesh and skin of radish (Raphanus sativus L.) fleshy root. The content of ascorbate decreased with root growth in both the flesh and skin. Expression of GDP-d-mannose pyrophosphorylase, GDP-d-mannose-3',5'-epimerase and d-galacturonate reductase were also decreased and correlated with ascorbate levels in the flesh. In the skin, the expression of GDP-d-mannose pyrophosphorylase and l-galactose dehydrogenase was correlated with ascorbate levels. These results suggested that ascorbate accumulation is affected mainly by biosynthesis rather than recycling in radish root, and the l-galactose pathway may be the major biosynthetic route of ascorbate, and moreover, the salvage pathway may also contribute to ascorbate accumulation. The data suggested that GDP-d-mannose pyrophosphorylase could play an important role in the regulation of ascorbate accumulation during radish fleshy taproot development
Beschreibung:	Date Completed 13.02.2014 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.05.041