Plant regeneration via somatic embryogenesis and shoot organogenesis from immature cotyledons of Camellia nitidissima Chi

Bibliographische Detailangaben
Veröffentlicht in:	Journal of plant physiology. - 1979. - 170(2013), 13 vom: 01. Sept., Seite 1202-11
1. Verfasser:	Lü, Jinfeng (VerfasserIn)
Weitere Verfasser:	Chen, Rong, Zhang, Muhan, da Silva, Jaime A Teixeira, Ma, Guohua
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Journal of plant physiology
Schlagworte:	Evaluation Study Journal Article Research Support, Non-U.S. Gov't 2,4-D 2,4-dichlorophenoxyacetic acid 6-benzylaminopurine Adventitious shoots AgNO(3) BAP Camellia nitidissima mehr... Embryogenic callus GA(3) IAA IBA Kt NAA NES Nodular embryogenic structures Rooting Somatic embryos TDZ Transplanting gibberellic acid indole-3-acetic acid indole-3-butyric acid kinetin nodular embryogenic structure silver nitrate thidiazuron α-naphthaleneacetic acid Plant Growth Regulators

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier GmbH. All rights reserved. Camellia nitidissima Chi (Theaceae) is a world-famous economic and ornamental plant with golden-yellow flowers. It has been classified as one of the rarest and most endangered plants in China. Our objective was to induce somatic embryogenesis, shoot organogenesis and plant regeneration for C. nitidissima. Three types of callus (whitish, reddish and yellowish) were induced from immature cotyledons on improved woody plant medium (WPM) with different plant growth regulators (PGRs). Among the callus, whitish callus was induced by 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and reddish and yellowish callus were induced by strongly active cytokinins, thidiazuron (TDZ) or 6-benzylaminopurine (BAP), singly or combined with weakly active auxin, α-naphthaleneacetic acid (NAA). The embryogenic callus could differentiate into somatic embryos, nodular embryogenic structures (large embryo-like structures) or adventitious shoots depending on the PGR used in WPM. BAP was best for adventitious buds and zeatin was best for somatic embryogenesis while kinetin (Kt) was best for the formation of nodular embryogenic structures. The three regeneration pathways often occurred in the same embryogenic callus clumps. Most shoots (80.0%) developed roots in WPM supplemented with 24.6 μM IBA and 0.3 μM NAA while 47.5% of somatic embryos could germinate directly and develop into plantlets on induction medium supplemented with 0.9 μM BAP and 0.1 μM NAA. The nodular embryogenic structures could be sub-cultured and cyclically developed in one of two differentiation pathways: shoot organogenesis or somatic embryogenesis. Plantlets derived from shoot buds rooted and somatic embryos germinated when transplanted into soil in a greenhouse; 66.7% of plantlets from shoot culture and 78.6% of plantlets from somatic embryos survived after 8 weeks' acclimatization
Beschreibung:	Date Completed 03.03.2014 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1618-1328
DOI:	10.1016/j.jplph.2013.03.019