An atypical pattern of accumulation of scopolamine and other tropane alkaloids and expression of alkaloid pathway genes in Hyoscyamus senecionis

An atypical pattern of accumulation of scopolamine and other tropane alkaloids and expression of alkaloid pathway genes in Hyoscyamus senecionis

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 70(2013) vom: 20. Sept., Seite 188-94
1. Verfasser: Dehghan, Esmail (VerfasserIn)
Weitere Verfasser: Shahriari Ahmadi, Farajollah, Ghotbi Ravandi, Elnaz, Reed, Darwin W, Covello, Patrick S, Bahrami, Ahmad Reza
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article 5-bromo-4-chloro-indolyl-β-d-galactopyranoside BSTFA Expression profile GC GC–MS Hyoscyamine 6β-hydroxylase (H6H) Hyoscyamus senecionis IPTG MS mehr... Murashige and Skoog ORF PMT Solanaceae TMCS TRI TRII Tropane alkaloids X-Gal bistrimethylsilyltrifluoroacetamide gas chromatography gas chromatography–mass spectrometry isopropyl β-d-1-thiogalactopyranoside open reading frame putrescine N-methyltransferase trimethylchlorosilane tropinone reductase I tropinone reductase II DNA, Complementary Plant Proteins Scopolamine DL48G20X8X Mixed Function Oxygenases EC 1.- Alcohol Oxidoreductases EC 1.1.- tropinone reductase EC 1.1.1.236 hyoscyamine (6S)-dioxygenase EC 1.14.11.11 Methyltransferases EC 2.1.1.- EC 2.1.1.53 Hyoscyamine PX44XO846X
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100 1 |a Dehghan, Esmail  |e verfasserin  |4 aut 
245 1 3 |a An atypical pattern of accumulation of scopolamine and other tropane alkaloids and expression of alkaloid pathway genes in Hyoscyamus senecionis 
264 1 |c 2013 
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500 |a Date Completed 13.02.2014 
500 |a Date Revised 10.03.2022 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Copyright © 2013 Elsevier Masson SAS. All rights reserved. 
520 |a A cDNA encoding hyoscyamine 6β-hydroxylase (H6H, EC 1.14.11.11), a bifunctional enzyme catalyzing the last two steps in the scopolamine biosynthetic pathway, was isolated from Hyoscyamus senecionis, a medicinal plant endemic to the Iranian plateau. Expression analysis indicates that Hsh6h is expressed in all tested organs of H. senecionis including roots, rhizomes, leaves, stems and flowers unlike the other tropane alkaloid producing species. In parallel to this, in leaves, levels of scopolamine, the product of H6H, were higher than the substrate hyoscyamine. These data suggest that not only does the conversion of hyoscyamine to scopolamine take place in the root, followed by translocation to aerial parts, but also accumulated hyoscyamine in the aerial parts may be converted to scopolamine by activity of HsH6H. Analysis of expression profiles of putrescine N-methyltransferase and tropinone reductase I and II genes also indicates the organ-independent expression of these genes. Here we also introduce H. senecionis as an important tropane alkaloid producing species with its thick underground parts as a source of hyoscyamine, while its leaves can be considered as a source of scopolamine 
650 4 |a Journal Article 
650 4 |a 5-bromo-4-chloro-indolyl-β-d-galactopyranoside 
650 4 |a BSTFA 
650 4 |a Expression profile 
650 4 |a GC 
650 4 |a GC–MS 
650 4 |a Hyoscyamine 6β-hydroxylase (H6H) 
650 4 |a Hyoscyamus senecionis 
650 4 |a IPTG 
650 4 |a MS 
650 4 |a Murashige and Skoog 
650 4 |a ORF 
650 4 |a PMT 
650 4 |a Solanaceae 
650 4 |a TMCS 
650 4 |a TRI 
650 4 |a TRII 
650 4 |a Tropane alkaloids 
650 4 |a X-Gal 
650 4 |a bistrimethylsilyltrifluoroacetamide 
650 4 |a gas chromatography 
650 4 |a gas chromatography–mass spectrometry 
650 4 |a isopropyl β-d-1-thiogalactopyranoside 
650 4 |a open reading frame 
650 4 |a putrescine N-methyltransferase 
650 4 |a trimethylchlorosilane 
650 4 |a tropinone reductase I 
650 4 |a tropinone reductase II 
650 7 |a DNA, Complementary  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Scopolamine  |2 NLM 
650 7 |a DL48G20X8X  |2 NLM 
650 7 |a Mixed Function Oxygenases  |2 NLM 
650 7 |a EC 1.-  |2 NLM 
650 7 |a Alcohol Oxidoreductases  |2 NLM 
650 7 |a EC 1.1.-  |2 NLM 
650 7 |a tropinone reductase  |2 NLM 
650 7 |a EC 1.1.1.236  |2 NLM 
650 7 |a hyoscyamine (6S)-dioxygenase  |2 NLM 
650 7 |a EC 1.14.11.11  |2 NLM 
650 7 |a Methyltransferases  |2 NLM 
650 7 |a EC 2.1.1.-  |2 NLM 
650 7 |a putrescine N-methyltransferase  |2 NLM 
650 7 |a EC 2.1.1.53  |2 NLM 
650 7 |a Hyoscyamine  |2 NLM 
650 7 |a PX44XO846X  |2 NLM 
700 1 |a Shahriari Ahmadi, Farajollah  |e verfasserin  |4 aut 
700 1 |a Ghotbi Ravandi, Elnaz  |e verfasserin  |4 aut 
700 1 |a Reed, Darwin W  |e verfasserin  |4 aut 
700 1 |a Covello, Patrick S  |e verfasserin  |4 aut 
700 1 |a Bahrami, Ahmad Reza  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 70(2013) vom: 20. Sept., Seite 188-94  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:70  |g year:2013  |g day:20  |g month:09  |g pages:188-94 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2013.05.007  |3 Volltext 
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