Induction of rice mutations by high hydrostatic pressure

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 70(2013) vom: 20. Sept., Seite 182-7
1. Verfasser:	Zhang, Wei (VerfasserIn)
Weitere Verfasser:	Liu, Xuncheng, Zheng, Feng, Zeng, Songjun, Wu, Kunlin, da Silva, Jaime A Teixeira, Duan, Jun
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't DMN DNA strand damage ENU HHP High hydrostatic pressure LMA Mutagenesis NMA mehr... Rice SDW UV dimethylnitrosamine ethylnitrosourea high hydrostatic pressure low-melting agarose normal-melting agarose sterile distilled water ultraviolet Water 059QF0KO0R

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier Masson SAS. All rights reserved. High hydrostatic pressure (HHP) is an extreme thermo-physical factor that affects the synthesis of DNA, RNA and proteins and induces mutagenesis in microorganisms. Our previous studies showed that exposure to 25-100 MPa HHP for 12 h retarded the germination and affected the viability of rice (Oryza sativa L.) seeds, increased the tolerance of rice plants to cold stress and altered gene expression patterns in germinating rice seeds. However, the mutagenic effect of HHP on rice remains unknown. In this study, exposure to 25, 50, 75 or 100 MPa for 12 h HHP could efficiently induce variation in rice plants. Furthermore, presoaking time and HHP strength during HHP treatment affected the efficiency of mutation. In addition, the Comet assay revealed that exposure to 25-100 MPa HHP for 12 h induced DNA strand breakage in germinating seeds and may have been the source of mutations. Our results suggest that HHP is a promising physical mutagen in rice breeding
Beschreibung:	Date Completed 13.02.2014 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.05.034