Genotyping of Streptococcus dysgalactiae strains isolated from Nile tilapia, Oreochromis niloticus (L.)

Genotyping of Streptococcus dysgalactiae strains isolated from Nile tilapia, Oreochromis niloticus (L.)

© 2013 John Wiley & Sons Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Journal of fish diseases. - 1998. - 37(2014), 5 vom: 16. Mai, Seite 463-9
1. Verfasser: Costa, F A A (VerfasserIn)
Weitere Verfasser: Leal, C A G, Leite, R C, Figueiredo, H C P
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Brazil Streptococcus dysgalactiae molecular epidemiology pulsed-field gel electrophoresis Bacterial Proteins Superoxide Dismutase EC 1.15.1.1
Beschreibung
Zusammenfassung:© 2013 John Wiley & Sons Ltd.
Streptococcus dysgalactiae is an emerging fish pathogen that is responsible for outbreaks of disease on fish farms around the world. Recently, this bacterium was associated with an outbreak at a Nile tilapia, Oreochromis niloticus (L.), farm in Brazil. The aim of this study was to evaluate the genetic diversity, best genotyping method and aspects of molecular epidemiology of S. dysgalactiae infections in Nile tilapia farms in Brazil. Twenty-one isolates from four farms located in different Brazilian states were characterized genetically using pulsed-field gel electrophoresis (PFGE), ERIC-PCR, REP-PCR and sodA gene sequencing. The discriminatory power of the different typing methods was compared using Simpson's index of diversity. Identical sodA gene sequences were obtained from all isolates, and ERIC-PCR and REP-PCR were unable to discriminate among the isolates. PFGE typing detected three different genetic patterns between the 21 strains evaluated; thus, it was the best genotyping method for use with this pathogen. The strains from Ceará State were genetically divergent from those from Alagoas State. The S. dysgalactiae isolates analysed in this study constituted a genetically diverse population with a clear association between geographical origin and genotype
Beschreibung:Date Completed 11.12.2014
Date Revised 30.09.2020
published: Print-Electronic
GENBANK: JX982805, JX982806, JX982807, JX982808, JX982809, JX982810, JX982811, JX982812, JX982813, JX982814, JX982815, JX982816, JX982817, JX982818, JX982819, JX982820, JX982821, JX982822, JX982823, JX982824, JX982825
Citation Status MEDLINE
ISSN:1365-2761
DOI:10.1111/jfd.12125