Genotypic differences in nitrate uptake, translocation and assimilation of two Chinese cabbage cultivars [Brassica campestris L. ssp. Chinensis (L.)

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 70(2013) vom: 18. Sept., Seite 14-20
1. Verfasser:	Tang, Yafang (VerfasserIn)
Weitere Verfasser:	Sun, Xuecheng, Hu, Chengxiao, Tan, Qiling, Zhao, Xiaohu
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Comparative Study Journal Article Research Support, Non-U.S. Gov't Assimilation Chinese cabbage GOGAT GS GSA Glu HATS mehr... LATS N NR NRA NiR Nitrate RT-PCR Translocation Uptake glutamate synthase glutamic acid glutamine synthetase glutamine synthetase activity high-affinity transport system low-affinity transport system nitrate reductase nitrate reductase activity nitrite reductase nitrogen real-time polymerase chain reaction Amino Acids Anion Transport Proteins Nitrates Plant Proteins Nitrate Reductase EC 1.7.99.4 Glutamate-Ammonia Ligase EC 6.3.1.2 Glucose IY9XDZ35W2 Nitrogen N762921K75

Beschreibung
Zusammenfassung:	Crown Copyright © 2013. Published by Elsevier Masson SAS. All rights reserved. A hydroponic trial was conducted to investigate genotypic differences in nitrate uptake, translocation and assimilation between low nitrate accumulator L18 and high accumulator H96 of Chinese cabbage [Brassica campestris L. ssp. Chinensis (L.)]. The results suggested that H96 could uptake more nitrate than L18 in the root but lower transport inside leaf cells and assimilation in the leaf. It was showed that root morphology parameters - length, surface area and volume of H96 were 18.0%, 31.6% and 46.5% higher than L18. Nitrate transporters NRT1.1 and NRT2.1 transcription levels were 41.6% and 269.6% higher than those of L18 respectively. NRT1.1 and NRT2.1 expression amount in leaf blade of two cultivars were opposite to in the root, L18 NRT1.1 and NRT2.1 expressions were 279.2% and 80.0% higher than H96. In addition, nitrate assimilation capacity of L18 was significantly higher than H96 in leaf. It was showed that Nitrate Reductase (NR; EC 1.7.1.1) activity and NIA expression level of L18 leaf were 234 0.4% and 105.4% higher than those of H96, Glutamine Synthetase (GS; EC 6.3.1.2) activity, Gln1 and Gln2 expression levels in the leaf blade of L18 were 43.9%, 331.5% and 124.8% higher than those of H96 respectively. Nitrate assimilation products-Glu, total amino acid, soluble protein content in the leaf of L18 were all significantly higher than those of H96. The results above suggested that nitrate accumulation differences were due to differential capacities to uptake, mechanisms for nitrate transport in leaves and assimilate nitrate. Comparing contribution of three aspects in nitrate accumulation, translocation and assimilation were contributed more in low nitrate concentration in the leaf blade
Beschreibung:	Date Completed 13.02.2014 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.04.027