Novel phenotypes related to the breeding of purple-fruited tomatoes and effect of peel extracts on human cancer cell proliferation

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 72(2013) vom: 02. Nov., Seite 125-33
1. Verfasser:	Mazzucato, Andrea (VerfasserIn)
Weitere Verfasser:	Willems, Daniela, Bernini, Roberta, Picarella, Maurizio E, Santangelo, Enrico, Ruiu, Fabrizio, Tilesi, Francesca, Soressi, Gian Piero
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide AN2 ANT1 ANTHOCYANIN1 ANTHOCYANIN2 Aft Anthocyanin fruit Anthocyanin mutants mehr... Antioxidants CHS FW GA(3) GM MTT OD SB Selection index Solanum lycopersicum L. Sun Black WT atroviolaceum atv chalcone synthase fresh weight genetically modified gibberellic acid optical density wild-type Anthocyanins Plant Extracts

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier Masson SAS. All rights reserved. The production of anthocyanins in the tomato (Solanum lycopersicum L.) fruit is normally absent or poor, but a number of mutants or introgression lines are known to increase anthocyanin levels in vegetative and reproductive tissues. Through conventional breeding, a genetic combination was obtained with the remarkable phenotype of a deep purple fruit pigmentation, due to an accumulation of anthocyanins on the peel. Such a genotype was named Sun Black (SB) as a consequence of its sensitivity to light induction. When characterized for morpho-agronomic traits, SB plants showed increased fertility. Purple fruits displayed an arrangement of the epicarp cells different from normal tomatoes, a feature that could account for different mechanical properties and shelf-life potential. The SB genotype and, to a lesser extent, its single mutant parents showed the capacity to accumulate anthocyanins in the seedling root when grown under light. This phenotype, which was greatly improved by the addition of sucrose to the germination medium, proved to be useful as selection index and gave new insights for in vitro production of anthocyanin extracts. To assess the nutraceutical potential of purple tomatoes, we tested the activity of SB skin extracts on the proliferation of two human cancer cells lines. Cell proliferation was significantly inhibited by SB extract in a dose-dependent manner. When the bioactivity of SB extracts was compared with that of other anthocyanin-containing fruits or vegetables, a significant "Extract*Line" interaction was evidenced, suggesting a crucial role for the extract composition in terms of anthocyanidins and other eventual cell growth-inhibiting compounds
Beschreibung:	Date Completed 13.06.2014 Date Revised 07.12.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2013.05.012