Upgraded ESRF BM29 beamline for SAXS on macromolecules in solution

Small-angle X-ray scattering (SAXS) measurements of proteins in solution are becoming increasingly popular with biochemists and structural biologists owing to the presence of dedicated high-throughput beamlines at synchrotron sources. As part of the ESRF Upgrade program a dedicated instrument for pe...

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Publié dans:Journal of synchrotron radiation. - 1994. - 20(2013), Pt 4 vom: 07. Juli, Seite 660-4
Auteur principal: Pernot, Petra (Auteur)
Autres auteurs: Round, Adam, Barrett, Ray, De Maria Antolinos, Alejandro, Gobbo, Alexandre, Gordon, Elspeth, Huet, Julien, Kieffer, Jerôme, Lentini, Mario, Mattenet, Muriel, Morawe, Christian, Mueller-Dieckmann, Christoph, Ohlsson, Staffan, Schmid, Werner, Surr, John, Theveneau, Pascal, Zerrad, Louiza, McSweeney, Sean
Format: Article en ligne
Langue:English
Publié: 2013
Accès à la collection:Journal of synchrotron radiation
Sujets:Journal Article automation and high throughput online HPLC proteins in solution small-angle X-ray scattering structural biology Proteins Solutions
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520 |a Small-angle X-ray scattering (SAXS) measurements of proteins in solution are becoming increasingly popular with biochemists and structural biologists owing to the presence of dedicated high-throughput beamlines at synchrotron sources. As part of the ESRF Upgrade program a dedicated instrument for performing SAXS from biological macromolecules in solution (BioSAXS) has been installed at the renovated BM29 location. The optics hutch has been equipped with new optical components of which the two principal elements are a fixed-exit double multilayer monochromator and a 1.1 m-long toroidal mirror. These new dedicated optics give improved beam characteristics (compared with the previous set-up on ID14-3) regarding the energy tunability, flux and focusing at the detector plane leading to reduced parasitic scattering and an extended s-range. User experiments on the beamline have been successfully carried out since June 2012. A description of the new BioSAXS beamline and the set-up characteristics are presented together with examples of obtained data 
650 4 |a Journal Article 
650 4 |a automation and high throughput 
650 4 |a online HPLC 
650 4 |a proteins in solution 
650 4 |a small-angle X-ray scattering 
650 4 |a structural biology 
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700 1 |a Round, Adam  |e verfasserin  |4 aut 
700 1 |a Barrett, Ray  |e verfasserin  |4 aut 
700 1 |a De Maria Antolinos, Alejandro  |e verfasserin  |4 aut 
700 1 |a Gobbo, Alexandre  |e verfasserin  |4 aut 
700 1 |a Gordon, Elspeth  |e verfasserin  |4 aut 
700 1 |a Huet, Julien  |e verfasserin  |4 aut 
700 1 |a Kieffer, Jerôme  |e verfasserin  |4 aut 
700 1 |a Lentini, Mario  |e verfasserin  |4 aut 
700 1 |a Mattenet, Muriel  |e verfasserin  |4 aut 
700 1 |a Morawe, Christian  |e verfasserin  |4 aut 
700 1 |a Mueller-Dieckmann, Christoph  |e verfasserin  |4 aut 
700 1 |a Ohlsson, Staffan  |e verfasserin  |4 aut 
700 1 |a Schmid, Werner  |e verfasserin  |4 aut 
700 1 |a Surr, John  |e verfasserin  |4 aut 
700 1 |a Theveneau, Pascal  |e verfasserin  |4 aut 
700 1 |a Zerrad, Louiza  |e verfasserin  |4 aut 
700 1 |a McSweeney, Sean  |e verfasserin  |4 aut 
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773 1 8 |g volume:20  |g year:2013  |g number:Pt 4  |g day:07  |g month:07  |g pages:660-4 
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