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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1111/nph.12358
|2 doi
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|a eng
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|a Kotur, Zorica
|e verfasserin
|4 aut
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|a Characterization of nitrite uptake in Arabidopsis thaliana
|b evidence for a nitrite-specific transporter
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 15.04.2014
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|a Date Revised 20.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
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|a Nitrite-specific plasma membrane transporters have been described in bacteria, algae and fungi, but there is no evidence of a nitrite-specific plasma membrane transporter in higher plants. We have used 13NO2(-) to characterize nitrite influx into roots of Arabidopsis thaliana. Hydroponically grown Arabidopsis mutants, defective in high-affinity nitrate transport, were used to distinguish between nitrate and nitrite uptake by means of the short-lived tracers 13NO2(-) and 13NO3(-). This approach allowed us to characterize a nitrite-specific transporter. The Atnar2.1-2 mutant, lacking a functional high-affinity nitrate transport system, is capable of nitrite influx that is constitutive and thermodynamically active. The corresponding fluxes conform to a rectangular hyperbola, exhibiting saturation at concentrations above 200 μM (Km = 185 μM and Vmax = 1.89 μmol g(-1) FW h(-1)). Nitrite influx via the putative nitrite transporter is not subject to competitive inhibition by nitrate but is downregulated after 6 h exposure to ammonium. These results signify the existence of a nitrite-specific transporter in Arabidopsis. This transporter enables Atnar2.1-2 mutants, which are incapable of sustained growth on low nitrate, to maintain significant growth on low nitrite. In wild-type plants, this nitrite flux may increase nitrogen acquisition and also participate in the induction of genes specifically induced by nitrite
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis
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|a Atnar2.1 mutant
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|a nitrate
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|a nitrite
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|a transporter
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|a Ammonium Compounds
|2 NLM
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|a Anion Transport Proteins
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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|a Nitrates
|2 NLM
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|a Nitrites
|2 NLM
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|a Nitrogen
|2 NLM
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|a N762921K75
|2 NLM
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| 700 |
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|a Siddiqi, Yaeesh M
|e verfasserin
|4 aut
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|a Glass, Anthony D M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1990
|g 200(2013), 1 vom: 01. Okt., Seite 201-210
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|x 1469-8137
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|g volume:200
|g year:2013
|g number:1
|g day:01
|g month:10
|g pages:201-210
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|u http://dx.doi.org/10.1111/nph.12358
|3 Volltext
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