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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1111/nph.12347
|2 doi
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|a pubmed24n1222.xml
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|a (NLM)23731343
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Hoser, Rafał
|e verfasserin
|4 aut
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| 245 |
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|a Nucleocytoplasmic partitioning of tobacco N receptor is modulated by SGT1
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.04.2014
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
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|a SGT1 (Suppressor of G2 allele of SKP1) is required to maintain plant disease Resistance (R) proteins with Nucleotide-Binding (NB) and Leucine-Rich Repeat (LRR) domains in an inactive but signaling-competent state. SGT1 is an integral component of a multi-protein network that includes RACK1, Rac1, RAR1, Rboh, HSP90 and HSP70, and in rice the Mitogen-Activated Protein Kinase (MAPK), OsMAPK6. Tobacco (Nicotiana tabacum) N protein, which belongs to the Toll-Interleukin Receptor (TIR)-NB-LRR class of R proteins, confers resistance to Tobacco Mosaic Virus (TMV). Following transient expression in planta, we analyzed the functional relationship between SGT1, SIPK - a tobacco MAPK6 ortholog - and N, using mass spectrometry, confocal microscopy and pathogen assays. Here, we show that tobacco SGT1 undergoes specific phosphorylation in a canonical MAPK target-motif by SIPK. Mutation of this motif to mimic SIPK phosphorylation leads to an increased proportion of cells displaying SGT1 nuclear accumulation and impairs N-mediated resistance to TMV, as does phospho-null substitution at the same residue. Forced nuclear localization of SGT1 causes N to be confined to nuclei. Our data suggest that one mode of regulating nucleocytoplasmic partitioning of R proteins is by maintaining appropriate levels of SGT1 phosphorylation catalyzed by plant MAPK
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a MAPK
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|a NB-LRR
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|a SGT1
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|a disease resistance
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|a nucleocytoplasmic shuttling
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|a protein phosphorylation
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|a tobacco (Nicotiana tabacum)
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Mitogen-Activated Protein Kinases
|2 NLM
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| 650 |
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|a EC 2.7.11.24
|2 NLM
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| 650 |
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|a SA-induced protein kinase
|2 NLM
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|a EC 2.7.11.24
|2 NLM
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| 700 |
1 |
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|a Żurczak, Marek
|e verfasserin
|4 aut
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| 700 |
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|a Lichocka, Małgorzata
|e verfasserin
|4 aut
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| 700 |
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|a Zuzga, Sabina
|e verfasserin
|4 aut
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| 700 |
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|a Dadlez, Michal
|e verfasserin
|4 aut
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| 700 |
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|a Samuel, Marcus A
|e verfasserin
|4 aut
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| 700 |
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|a Ellis, Brian E
|e verfasserin
|4 aut
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| 700 |
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|a Stuttmann, Johannes
|e verfasserin
|4 aut
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| 700 |
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|a Parker, Jane E
|e verfasserin
|4 aut
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| 700 |
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|a Hennig, Jacek
|e verfasserin
|4 aut
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| 700 |
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|a Krzymowska, Magdalena
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 200(2013), 1 vom: 19. Okt., Seite 158-171
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:200
|g year:2013
|g number:1
|g day:19
|g month:10
|g pages:158-171
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|u http://dx.doi.org/10.1111/nph.12347
|3 Volltext
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|d 200
|j 2013
|e 1
|b 19
|c 10
|h 158-171
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