Transcription factor MYB46 is an obligate component of the transcriptional regulatory complex for functional expression of secondary wall-associated cellulose synthases in Arabidopsis thaliana

Transcription factor MYB46 is an obligate component of the transcriptional regulatory complex for functional expression of secondary wall-associated cellulose synthases in Arabidopsis thaliana

Copyright © 2013 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 170(2013), 15 vom: 15. Okt., Seite 1374-8
1. Verfasser: Kim, Won-Chan (VerfasserIn)
Weitere Verfasser: Kim, Joo-Yeol, Ko, Jae-Heung, Kim, Jungmook, Han, Kyung-Hwan
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Arabidopsis CDS CESA Cellulose synthase MYB46 Secondary wall TF mehr... Transcription factor cellulose synthase A coding sequence transcription factor Arabidopsis Proteins Myb46 protein, Arabidopsis Transcription Factors Glucosyltransferases EC 2.4.1.- cellulose synthase
Beschreibung
Zusammenfassung:Copyright © 2013 Elsevier GmbH. All rights reserved.
Cellulose, the most abundant biopolymer on Earth, is a central component in plant cell walls and highly abundant (up to 50%) in the secondary walls. In Arabidopsis thaliana, the cellulose biosynthesis in the secondary walls is catalyzed by three cellulose synthases CESA4, CESA7 and CESA8. The transcription factor MYB46 and its close homolog MYB83 directly regulate the expression of the three secondary wall cellulose synthases (CESAs). However, it is not known whether MYB46 is the necessary regulator for functional expression of the secondary wall CESAs or one of the multiple transcriptional factors involved in the transcriptional regulatory program. To address this question, we used a series of genetic complementation experiments of the cesa knock-out mutants with the CESA coding sequence driven by either native- or mutated promoter of the genes. The mutant promoters have two nucleotide point mutations in the MYB46 binding cis element (M46RE) such that MYB46 cannot bind to the promoter, while the binding of other known secondary wall transcription factors is not affected. The mutant complementation results showed that MYB46 is essential to restore normal phenotype from the cesa mutants. We conclude that MYB46 is an obligate component of the transcriptional regulatory complex toward the commitment of secondary wall cellulose synthesis in Arabidopsis
Beschreibung:Date Completed 13.03.2014
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1618-1328
DOI:10.1016/j.jplph.2013.04.012