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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1021/la400871c
|2 doi
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|a pubmed25n0759.xml
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|a eng
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| 100 |
1 |
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|a Amorim, Sara
|e verfasserin
|4 aut
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|a Interactions between exogenous FGF-2 and sulfonic groups
|b in situ characterization and impact on the morphology of human adipose-derived stem cells
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|c 2013
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 03.02.2014
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|a Date Revised 29.08.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a FGF-2 is often used as a supplement to stem cells culture medium aiming at preserving their self-renewal capacity and plasticity through the passages. However, little is known on the influence of the underlying substrate in these interactions. In this study, we have used mixed self-assembled monolayers with different ratios of -SO3H and -OH tail groups to investigate the influence of substrate properties (e.g., charge) on the FGF-2 adsorption and activity. QCM-D data demonstrated that, in the presence of -OH groups, the quantity of the adsorbed FGF-2 is proportional to the percentage of surface -SO3H groups. The bioactivity of the adsorbed FGF-2 follows the same tendency as demonstrated by its interactions with anti-FGF-2. Surprisingly, the adlayer of FGF-2 formed on the surface containing only SO3H-tailed SAMs was similar to the surface with 25% of -SO3H groups, demonstrating that FGF-2 adsorption is not solely driven by electrostatic interactions. We related these results with changes in the morphology of adipose-derived stem cells (ASCs) cultured on the same surfaces
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fibroblast Growth Factor 2
|2 NLM
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|a 103107-01-3
|2 NLM
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| 700 |
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|a Pires, Ricardo A
|e verfasserin
|4 aut
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|a da Costa, Diana Soares
|e verfasserin
|4 aut
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|a Reis, Rui L
|e verfasserin
|4 aut
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|a Pashkuleva, Iva
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 29(2013), 25 vom: 25. Juni, Seite 7983-92
|w (DE-627)NLM098181009
|x 1520-5827
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|g volume:29
|g year:2013
|g number:25
|g day:25
|g month:06
|g pages:7983-92
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|u http://dx.doi.org/10.1021/la400871c
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