Quantum dots self assembly based interface for blood cancer detection
Results of the studies related to fabrication of sensitive electrochemical biosensor using an interface based on quantum dots (QDs) self-assembly is reported. The QDs assembly is sought to provide improved fundamental characteristics to the electrode interface in terms of electroactive surface area,...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 29(2013), 27 vom: 09. Juli, Seite 8753-62 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Cadmium Compounds Oligonucleotide Probes Tellurium NQA0O090ZJ cadmium telluride STG188WO13 |
| Zusammenfassung: | Results of the studies related to fabrication of sensitive electrochemical biosensor using an interface based on quantum dots (QDs) self-assembly is reported. The QDs assembly is sought to provide improved fundamental characteristics to the electrode interface in terms of electroactive surface area, diffusion coefficient, and electron transfer kinetics. This QDs modified electrode has been utilized to serve as a transducer surface for covalent immobilization of chronic myelogenous leukemia (CML) specific probe oligonucleotide, designed from the BCR-ABL fusion gene. The electrochemical characteristics of this biosensor toward various designed synthetic oligonucleotides reveal a significant enhancement in its mismatch discrimination capability compared to the biosensing assay without QDs under similar experimental conditions. The sensing characteristics of this biosensor offer a potential for detection of target oligonucleotide at a concentration as low as 1.0 pM. Furthermore, the PCR-amplified CML-positive patient samples with various BCR-ABL transcript ratios can be electrochemically distinguished from healthy samples, indicating promising application of the QDs based biosensor for clinical investigations |
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| Beschreibung: | Date Completed 07.02.2014 Date Revised 25.11.2016 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la401431q |