MtQRRS1, an R-locus required for Medicago truncatula quantitative resistance to Ralstonia solanacearum
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
| Veröffentlicht in: | The New phytologist. - 1990. - 199(2013), 3 vom: 18. Aug., Seite 758-72 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Medicago truncatula Ralstonia solanacearum bacterial wilt mapping quantitative resistance quantitative trait locus (QTL) root pathogen |
| Zusammenfassung: | © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust. Ralstonia solanacearum is a major soilborne pathogen that attacks > 200 plant species, including major crops. To characterize MtQRRS1, a major quantitative trait locus (QTL) for resistance towards this bacterium in the model legume Medicago truncatula, genetic and functional approaches were combined. QTL analyses together with disease scoring of heterogeneous inbred families were used to define the locus. The candidate region was studied by physical mapping using a bacterial artificial chromosome (BAC) library of the resistant line, and sequencing. In planta bacterial growth measurements, grafting experiments and gene expression analysis were performed to investigate the mechanisms by which this locus confers resistance to R. solanacearum. The MtQRRS1 locus was localized to the same position in two recombinant inbred line populations and was narrowed down to a 64 kb region. Comparison of parental line sequences revealed 15 candidate genes with sequence polymorphisms, but no evidence of differential gene expression upon infection. A role for the hypocotyl in resistance establishment was shown. These data indicate that the quantitative resistance to bacterial wilt conferred by MtQRRS1, which contains a cluster of seven R genes, is shared by different accessions and may act through intralocus interactions to promote resistance |
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| Beschreibung: | Date Completed 29.01.2014 Date Revised 30.09.2020 published: Print-Electronic GENBANK: JX457607 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.12299 |