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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2013.03.020
|2 doi
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|a pubmed24n0757.xml
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|a eng
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|a Quarta, Angela
|e verfasserin
|4 aut
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| 245 |
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|a Isolation of a polyphenol oxidase (PPO) cDNA from artichoke and expression analysis in wounded artichoke heads
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 22.01.2014
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|a Date Revised 31.03.2022
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|a published: Print-Electronic
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|a GENBANK: KC342809
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier Masson SAS. All rights reserved.
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|a The polyphenol oxidase (PPO) enzyme, which can catalyze the oxidation of phenolics to quinones, has been reported to be involved in undesirable browning in many plant foods. This phenomenon is particularly severe in artichoke heads wounded during the manufacturing process. A full-length cDNA encoding for a putative polyphenol oxidase (designated as CsPPO) along with a 1432 bp sequence upstream of the starting ATG codon was characterized for the first time from [Cynara cardunculus var. scolymus (L.) Fiori]. The 1764 bp CsPPO sequence encodes a putative protein of 587 amino acids with a calculated molecular mass of 65,327 Da and an isoelectric point of 5.50. Analysis of the promoter region revealed the presence of cis-acting elements, some of which are putatively involved in the response to light and wounds. Expression analysis of the gene in wounded capitula indicated that CsPPO was significantly induced after 48 h, even though the browning process had started earlier. This suggests that the early browning event observed in artichoke heads was not directly related to de novo mRNA synthesis. Finally, we provide the complete gene sequence encoding for polyphenol oxidase and the upstream regulative region in artichoke
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|a Journal Article
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|a DNA, Complementary
|2 NLM
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Catechol Oxidase
|2 NLM
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| 650 |
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|a EC 1.10.3.1
|2 NLM
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| 700 |
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|a Mita, Giovanni
|e verfasserin
|4 aut
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|a Durante, Miriana
|e verfasserin
|4 aut
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|a Arlorio, Marco
|e verfasserin
|4 aut
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|a De Paolis, Angelo
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 68(2013) vom: 07. Juli, Seite 52-60
|w (DE-627)NLM098178261
|x 1873-2690
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|g volume:68
|g year:2013
|g day:07
|g month:07
|g pages:52-60
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|u http://dx.doi.org/10.1016/j.plaphy.2013.03.020
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