Improved native isolation of endogenous Protein A-tagged protein complexes
Here we report a modified peptide reagent useful for the rapid, native elution of protein complexes containing a Protein-A-tagged component. We tested this reagent for the elution of tagged endogenous protein complexes from yeast (Nup53p/Nup170p dimer; Nup1p/Kap95p/Kap60p trimer; pentameric GINS com...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 54(2013), 4 vom: 24. Apr., Seite 213-6
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| 1. Verfasser: |
LaCava, John
(VerfasserIn) |
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| Weitere Verfasser: |
Chandramouli, Nagarajan,
Jiang, Hua,
Rout, Michael P |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Bacterial Proteins
Fungal Proteins
Immunoglobulin G
Indicators and Reagents
Multiprotein Complexes
Peptides
Staphylococcal Protein A
Polyethylene Glycols
3WJQ0SDW1A |
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