Functional study of TCP23 in Arabidopsis thaliana during plant development

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 67(2013) vom: 07. Juni, Seite 120-5
1. Verfasser: Balsemão-Pires, Emilia (VerfasserIn)
Weitere Verfasser: Andrade, Leonardo R, Sachetto-Martins, Gilberto
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Flowering Plant development Subcellular localization TCP family Transcriptional factor Arabidopsis Proteins DNA, Bacterial T-DNA
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520 |a The TCP class of genes is found only in plants and is represented by the first three identified genes: teosinte branched 1, cycloidea and pcf. Members belonging to this class are important regulators of plant growth, development and control multiple traits in diverse plant species, including flower and petal asymmetry, plant architecture, leaf morphogenesis and senescence, embryo growth and circadian rhythm. Here we described a member of the TCP-P subfamily called AtTCP23. Using qRT-PCR we present evidence that AtTCP23 is ubiquitously express in all organs examined. To ascertain AtTCP23 localization, we fused GFP at the C-terminal position and analyzed stable expression by confocal microscopy. Transgenic lines harboring the full-length protein (OxTCP23:GFP) seems to accumulate GFP in the nucleus. In order to analyze AtTCP23 function, we obtained a T-DNA insertional line and developed AtTCP23 over-expression (OxTCP23) lines. Phenotypic analysis indicates that tcp23-1 knockout line has an early-flowering phenotype while overexpression lines (OxTCP23 and OxTCP23:eGFP) presents opposite phenotype. Besides that those lines have leaf morphology alteration, pale leaf borders and smaller roots. Thus we propose in this study that AtTCP23 may be involved in flowering time control and plant development 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Flowering 
650 4 |a Plant development 
650 4 |a Subcellular localization 
650 4 |a TCP family 
650 4 |a Transcriptional factor 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a DNA, Bacterial  |2 NLM 
650 7 |a T-DNA  |2 NLM 
700 1 |a Andrade, Leonardo R  |e verfasserin  |4 aut 
700 1 |a Sachetto-Martins, Gilberto  |e verfasserin  |4 aut 
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