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231224s2013 xx |||||o 00| ||eng c |
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7 |
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|a 10.1021/la400280x
|2 doi
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|a pubmed24n0754.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Hardin, James O
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Altering colloidal surface functionalization using DNA encapsulated inside monodisperse gelatin microsphere templates
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 12.12.2013
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|a Date Revised 07.05.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Soluble oligonucleotides are typically introduced to bulk solution to promote hybridization activity on DNA-functionalized surfaces. Here, an alternative approach is explored by encapsulating secondary target strands inside semipermeable colloidal satellite assemblies, then triggering their release at 37 °C for subsequent surface hybridization activity. To prepare DNA-loaded satellite assemblies, uniform gelatin microspheres are fabricated using microfluidics, loaded with 15 base-long secondary DNA targets, capped with a polyelectrolyte bilayer, and finally coated with a monolayer of polystyrene microspheres functionalized with duplexes comprised of immobilized probes and soluble, 13 base-long hybridization partner strands. Once warmed to 37 °C, secondary DNA targets are released from the gelatin template and then competitively displace the shorter, original hybridization partners on the polystyrene microspheres
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Capsules
|2 NLM
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|a Colloids
|2 NLM
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|a Gelatin
|2 NLM
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|a 9000-70-8
|2 NLM
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Fernandez-Nieves, Alberto
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martinez, Carlos J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Milam, Valeria T
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 29(2013), 18 vom: 07. Mai, Seite 5534-9
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:29
|g year:2013
|g number:18
|g day:07
|g month:05
|g pages:5534-9
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|u http://dx.doi.org/10.1021/la400280x
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