Apoptosis of human umbilical vein endothelial cells induced by IgA1 isolated from Henoch-Schönlein purpura patients
OBJECTIVE: To observe the effect of apoptosis of human umbilical vein endothelial cells (HUVEC) induced by IgA1 from Henoch-Schönlein purpura (HSP) patients
| Veröffentlicht in: | Zhonghua er ke za zhi = Chinese journal of pediatrics. - 1960. - 51(2013), 1 vom: 15. Jan., Seite 42-6 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | Zhonghua er ke za zhi = Chinese journal of pediatrics |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't FASLG protein, human Fas Ligand Protein Immunoglobulin A RNA, Messenger CASP3 protein, human EC 3.4.22.- Caspase 3 |
| Zusammenfassung: | OBJECTIVE: To observe the effect of apoptosis of human umbilical vein endothelial cells (HUVEC) induced by IgA1 from Henoch-Schönlein purpura (HSP) patients METHOD: HUVEC were cultured in 3 different conditional media with IgA1 from HSP patients, normal healthy children and simply the cell culture medium. Serum IgA1 was purified by jacalin affinity chromatography, rates of apoptosis in HUVEC cells at different concentration and different times after incubation with IgA1 were determined by TUNEL method and flow cytometry. Real-time PCR and Western blot methods were used to detect the expression of caspase-3 and Fas, respectively RESULT: Apoptosis rate of HUVEC by IgA1 isolated from HSP patients were significantly higher than that of the blank control [(14.77 ± 2.23)% vs. (2.25 ± 0.77)%, P < 0.01] and the apoptosis rate of HUVEC induced by IgA1 from normal healthy children was higher than that of blank control [(7.97 ± 1.48)% vs. (2.25 ± 0.77)%, P < 0.01]. The apoptosis rate of HUVEC induced by IgA1 from HSP was time and concentration-dependent. Moreover IgA1 isolated from HSP patients could significantly increase the caspase-3 and Fas expression (P < 0.01) CONCLUSION: The IgA1 from HSP patients could induce the apoptosis of HUVEC, which might be related to the progression of HSP |
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| Beschreibung: | Date Completed 19.03.2014 Date Revised 03.12.2021 published: Print Citation Status MEDLINE |
| ISSN: | 0578-1310 |