The survival of cefazolin-resistant bacteria in mesophilic co-digestion of dairy manure and waste milk

The use of cefazolin to treat mastitic cows leads to cefazolin residues in milk and manure. This is responsible for the high occurrence of cefazolin resistant bacteria (CRB) in waste and the environment. Anaerobic digestion is considered to have the potential to reduce antibiotic-resistant bacteria...

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Veröffentlicht in:Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA. - 1991. - 31(2013), 8 vom: 19. Aug., Seite 843-8
1. Verfasser: Beneragama, Nilmini (VerfasserIn)
Weitere Verfasser: Moriya, Yusuke, Yamashiro, Takaki, Iwasaki, Masahiro, Lateef, Suraju A, Ying, Chun, Umetsu, Kazutaka
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA
Schlagworte:Journal Article Cefazolin anaerobic digestion cefazolin-resistant bacteria co-digestion dairy manure waste milk Anti-Bacterial Agents Manure IHS69L0Y4T
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520 |a The use of cefazolin to treat mastitic cows leads to cefazolin residues in milk and manure. This is responsible for the high occurrence of cefazolin resistant bacteria (CRB) in waste and the environment. Anaerobic digestion is considered to have the potential to reduce antibiotic-resistant bacteria present in waste that results from concentrated animal feeding operations. Thus, the objective of this study was to investigate the survival of CRB and the digester performance in mesophilic co-digestion of dairy manure and waste milk. The experiment was carried out using three digester compositions: 100% slurry (slurry), 50% slurry + 50% manure (manure mixture) and 50% slurry + 45% manure + 5% waste milk (milk mixture) in batch digesters of 1 l with a working volume of 800 ml in triplicate at 37°C for 34 days. The daily biogas production in each digester, and methane (CH4) and carbon dioxide compositions in the gas were determined. The population densities of total culturable bacteria (TCB) and CRB were determined by plate counts on agar media at day 0, 10, 20 and 34 of digestion. Milk mixture produced the highest (P < 0.05) daily and cumulative total and CH4 gas. The maximum percentage reductions of TCB and CRB in manure and milk mixture was observed at day 20, the values being 96.2%, 96.0% and 99.8% and 99.8% respectively. Final volatile fatty acids (VFA) and pH values of the digesters confirmed the digester stability. Based on the findings, mesophilic anaerobic digestion can be considered a potent method to avoid the dissemination of CRB in nature 
650 4 |a Journal Article 
650 4 |a Cefazolin 
650 4 |a anaerobic digestion 
650 4 |a cefazolin-resistant bacteria 
650 4 |a co-digestion 
650 4 |a dairy manure 
650 4 |a waste milk 
650 7 |a Anti-Bacterial Agents  |2 NLM 
650 7 |a Manure  |2 NLM 
650 7 |a Cefazolin  |2 NLM 
650 7 |a IHS69L0Y4T  |2 NLM 
700 1 |a Moriya, Yusuke  |e verfasserin  |4 aut 
700 1 |a Yamashiro, Takaki  |e verfasserin  |4 aut 
700 1 |a Iwasaki, Masahiro  |e verfasserin  |4 aut 
700 1 |a Lateef, Suraju A  |e verfasserin  |4 aut 
700 1 |a Ying, Chun  |e verfasserin  |4 aut 
700 1 |a Umetsu, Kazutaka  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA  |d 1991  |g 31(2013), 8 vom: 19. Aug., Seite 843-8  |w (DE-627)NLM098164791  |x 1096-3669  |7 nnns 
773 1 8 |g volume:31  |g year:2013  |g number:8  |g day:19  |g month:08  |g pages:843-8 
856 4 0 |u http://dx.doi.org/10.1177/0734242X13477717  |3 Volltext 
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