A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

MicroRNAs (miRNAs) are short (~22 nucleotides), non-coding RNA molecules that post-transcriptionally regulate gene expression. As the miRNA field is still in its relative infancy, there is currently a lack of consensus regarding optimal methodologies for miRNA quantification, data analysis and data...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 54(2013), 3 vom: 01. März, Seite 155-64
1. Verfasser: Redshaw, Nicholas (VerfasserIn)
Weitere Verfasser: Wilkes, Timothy, Whale, Alexandra, Cowen, Simon, Huggett, Jim, Foy, Carole A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't MicroRNAs RNA, Plant