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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2013.01.018
|2 doi
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|a pubmed24n0752.xml
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|a (DE-627)NLM225573180
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|a (NLM)23466745
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|a (PII)S0981-9428(13)00038-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Yang
|e verfasserin
|4 aut
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| 245 |
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|a A cotton LIM domain-containing protein (GhWLIM5) is involved in bundling actin filaments
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.09.2013
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|a Date Revised 16.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier Masson SAS. All rights reserved.
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|a LIM-domain proteins play important roles in cellular processes in eukaryotes. In this study, a LIM protein gene, GhWLIM5, was identified in cotton. Quantitative RT-PCR analysis showed that GhWLIM5 was expressed widely in different cotton tissues and had a peak in expression during fiber elongation. GFP fluorescence assay revealed that cotton cells expressing GhWLIM5:eGFP fusion gene displayed a network distribution of eGFP fluorescence, suggesting that GhWLIM5 protein is mainly localized to the cell cytoskeleton. When GhWLIM5:eGFP transformed cells were stained with rhodamine-phalloidin there was consistent overlap in eGFP and rhodamine-palloidin signals, demonstrating that GhWLIM5 protein is colocalized with the F-actin cytoskeleton. In addition, high-speed cosedimentation assay verified that GhWLIM5 directly bound actin filaments, while low cosedimentation assay and microscopic observation indicated that GhWLIM5 bundled F-actin in vitro. Increasing amounts of GhWLIM5 protein were able to protect F-actin from depolymerization in vitro in the presence of Lat B (an F-actin depolymerizer). Our results contribute to a better understanding of the biochemical role of GhWLIM5 in modulating the dynamic F-actin network in cotton
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Actins
|2 NLM
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|a LIM Domain Proteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Recombinant Fusion Proteins
|2 NLM
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|a Rhodamines
|2 NLM
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|a enhanced green fluorescent protein
|2 NLM
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|a rhodamine-phalloidin
|2 NLM
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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| 650 |
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|a 147336-22-9
|2 NLM
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| 650 |
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|a Phalloidine
|2 NLM
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| 650 |
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|a 17466-45-4
|2 NLM
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| 700 |
1 |
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|a Jiang, Jia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Lan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Xiu-Lan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Na-Na
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Deng-Di
|e verfasserin
|4 aut
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| 700 |
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|a Li, Xue-Bao
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 66(2013) vom: 15. Mai, Seite 34-40
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:66
|g year:2013
|g day:15
|g month:05
|g pages:34-40
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|u http://dx.doi.org/10.1016/j.plaphy.2013.01.018
|3 Volltext
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|d 66
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